Cloned (Comment) | Organism |
---|---|
a hexahistidine affinity-tagged NS5B fusion protein is expressed with recombinant baculoviruses in insect cells | Hepacivirus C |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a hexahistidine affinity-tagged NS5B fusion protein is expressed with recombinant baculoviruses in insect cells | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | at high concentrations | Hepacivirus C | |
Zn2+ | complete inhibition at concentrations above 1.25 mM ZnCl2 | Hepacivirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
CTP | pH 7.5, 22°C, HCV 3'-end as template | Hepacivirus C | |
0.00054 | - |
GTP | pH 7.5, 22°C, HCV 3'-end as template | Hepacivirus C | |
0.0029 | - |
GTP | pH 7.5, 22°C, HCV 3'-end as template | Hepacivirus C | |
0.00297 | - |
GTP | pH 7.5, 22°C, poly(C)/oligo(G) as template | Hepacivirus C | |
0.0108 | - |
ATP | pH 7.5, 22°C, HCV 3'-end as template | Hepacivirus C | |
0.022 | - |
UTP | pH 7.5, 22°C, poly(A)/oligo(U) as template | Hepacivirus C |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | optimal activity at 10 mM | Hepacivirus C | |
Mg2+ | absolutely dependent on, optimal activity at 12.5 mM MgCl2 | Hepacivirus C | |
Mn2+ | can replace for Mg2+, optimal concentration is 7-9 mM MnCl2 | Hepacivirus C | |
NaCl | optimal activity at 80-100 mM | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
a hexahistidine affinity-tagged NS5B fusion protein expressed with recombinant baculoviruses in insect cells | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
CTP + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
GTP + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme is able to copy long heteropolymeric templates with an elongation rate of 150-200 nucleotides per min at 22°C | Hepacivirus C |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Hepacivirus C |