Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | concentrations of 1.0 mM, 0.5 mM and 0.05 mM in the presence of Mg2+ inhibit activity by 75%, 50% and 25%, respectively | West Nile virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ is the preferred divalent cation, optimal activity at 3-30 mM | West Nile virus | |
Mn2+ | 0.05 mM, 40% of the activity compared to reaction with 3 mM Mg2+. 5.0 mM, 70% of the activity compared to the reaction with 3 mM Mg2+, Mn2+ is present as the sole divalent cation | West Nile virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
West Nile virus | - |
BHK cells infected with | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
West Nile virus | diphosphate + RNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | - |
West Nile virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in Hepes-NaOH buffer or Tris hydrochloride buffer | West Nile virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.4 | pH 7.6: about 80% of maximal activity, pH 8.4: about 65% of maximal activity, in Tris hydrochloride buffer | West Nile virus |