Literature summary for 2.7.7.47 extracted from

Kim, C.; Hesek, D.; Zajicek, J.; Vakulenko, S.B.; Mobashery, S.
Characterization of the bifunctional aminoglycoside-modifying enzyme ANT(3)-Ii/AAC(6)-IId from Serratia marcescens (2006), Biochemistry, 45, 8368-8377.

Search for more literature for 2.7.7.47

Cloned(Commentary)

Cloned (Comment)	Organism
cloned in Escherichia coli BL21	Serratia marcescens

Inhibitors

Inhibitors	Comment	Organism	Structure
9-O-(adenosine-5'-phophoryl)spectinomycin	product inhibition of ANT(3'')-Ii domain	Serratia marcescens
AMP-CPP	dead-end inhibition of ANT(3'')-Ii domain	Serratia marcescens
kanamycin A	dead-end inhibition of ANT(3'')-Ii domain	Serratia marcescens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0013	-	streptomycin	kinetic parameter of the ANT(3'')-Ii domain of the enzyme	Serratia marcescens
0.0014	-	spectinomycin	kinetic parameter of the ANT(3'')-Ii domain of the enzyme	Serratia marcescens
0.014	-	ATP	kinetic parameter of the ANT(3'')-Ii domain of the enzyme	Serratia marcescens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	assay with 15 mM MgCl2	Serratia marcescens

Organism

Organism	UniProt	Comment	Textmining
Serratia marcescens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE anion-exchange column and gentamycin affinity column	Serratia marcescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + spectinomycin	-	Serratia marcescens	diphosphate + 9-adenylylspectinomycin	-	?
ATP + streptomycin	bifunctional enzyme, adenylation of aminoglycoside antibiotics takes place at the ANT(3'')-Ii domain, aceylation of aminoglycoside antibiotics takes place at the AAC(6')-domain	Serratia marcescens	diphosphate + 3''-adenylylstreptomycin	-	?
additional information	adenyltransferase domain is highly specific for spectinomycin and streptomycin and catalyzes the reaction by a Theorell-Chance kinetic mechanism, where ATP binds to the enzyme prior to the aminoglycoside and the modified antibiotic is the last product to be released	Serratia marcescens	?	-	?

Synonyms

Synonyms	Comment	Organism
ANT(3'')-Ii/AAC(6')-IId	-	Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	assay is performed at room temperature	Serratia marcescens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.5	-	ATP	turnover number of ANT(3'')-Ii domain	Serratia marcescens
0.5	-	spectinomycin	turnover number of ANT(3'')-Ii domain	Serratia marcescens
0.6	-	streptomycin	turnover number of ANT(3'')-Ii domain	Serratia marcescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Serratia marcescens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.004	-	kanamycin A	dead-end inhibition of ANT(3'')-Ii domain, substrate 0.02 mM spectinomycin, competitive inhibition	Serratia marcescens
0.01	-	kanamycin A	Kii = 0.01 mM, dead-end inhibition of ANT(3'')-Ii domain, substrate 0.1 mM ATP, uncompetitive inhibition	Serratia marcescens
0.017	-	AMP-CPP	dead-end inhibition of ANT(3'')-Ii domain, substrate 0.1 mM ATP, competitive inhibition	Serratia marcescens
0.021	-	AMP-CPP	dead-end inhibition of ANT(3'')-Ii domain, substrate 0.02 mM spectinomycin, Kii = 0.031 mM, noncompetitive/mixed inhibition	Serratia marcescens
0.076	-	9-O-(adenosine-5'-phophoryl)spectinomycin	Kii = 0.089, product inhibition of ANT(3'')-Ii domain, substrate 0.02 mM spectinomycin, noncompetitive/mixed inhibition	Serratia marcescens
0.093	-	9-O-(adenosine-5'-phophoryl)spectinomycin	product inhibition of ANT(3'')-Ii domain, substrate 0.1 mM ATP, competitive inhibition	Serratia marcescens

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Release 2023.1 (January 2023)