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Literature summary for 2.7.7.46 extracted from

  • Bacot-Davis, V.R.; Bassenden, A.V.; Sprules, T.; Berghuis, A.M.
    Effect of solvent and protein dynamics in ligand recognition and inhibition of aminoglycoside adenyltransferase 2''-Ia (2017), Protein Sci., 26, 1852-1863 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.46

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
D44A the mutation causes a 4fold decrease in aminoglycoside adenylation compared to the wild type enzyme Klebsiella pneumoniae
I129A the mutation causes a 3fold decrease in aminoglycoside adenylation compared to the wild type enzyme Klebsiella pneumoniae
Y135A the mutation causes a 1.6fold decrease in aminoglycoside adenylation compared to the wild type enzyme Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 100 mM used in assay conditions Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae P0AE05
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gentamicin
-
 Klebsiella pneumoniae diphosphate + 2''-adenylylgentamicin
-
 ?
ATP + kanamycin A
-
 Klebsiella pneumoniae diphosphate + 2''-adenylylkanamycin A
-
 ?
ATP + kanamycin B
-
 Klebsiella pneumoniae diphosphate + 2''-adenylylkanamycin B
-
 ?
ATP + tobramycin A
-
 Klebsiella pneumoniae diphosphate + 2''-adenylyltobramycin
-
 ?

Synonyms

Synonyms Comment Organism
aminoglycoside adenyltransferase 2''-Ia
-
 Klebsiella pneumoniae
ANT(2'')-Ia
-
 Klebsiella pneumoniae