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Literature summary for 2.7.7.46 extracted from
- Purification and properties of gentamicin nucleotidyltransferase from Escherichia coli: nucleotide specificity, pH optimum, and the separation of two electrophoretic variants (1984), Arch. Biochem. Biophys., 230, 250-263.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| MgATP2- | metal chelates being the true substrates, free nucleotides being inhibitors | Escherichia coli |  |
| MgCTP2- | metal chelates being the true substrates, free nucleotides being inhibitors | Escherichia coli | |
| MgdATP2- | metal chelates being the true substrates, free nucleotides being inhibitors | Escherichia coli | |
| MgdCTP2- | metal chelates being the true substrates, free nucleotides being inhibitors | Escherichia coli | |
| MgdGTP2- | metal chelates being the true substrates, free nucleotides being inhibitors | Escherichia coli | |
| MgdTTP2- | metal chelates being the true substrates, free nucleotides being inhibitors | Escherichia coli | |
| MgGTP2- | metal chelates being the true substrates, free nucleotides being inhibitors | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00276 | - |
tobramycin | pH 9.0, 25°C | Escherichia coli | |
| 0.03 | - |
dATP | pH 9.0, 25°C | Escherichia coli | |
| 0.03 | - |
dGTP | pH 9.0, 25°C | Escherichia coli | |
| 0.03 | - |
dATP | dATP in form of MgdATP2- | Escherichia coli | |
| 0.03 | - |
dGTP | dGTP in form of MgdGTP2- | Escherichia coli | |
| 0.09 | - |
dCTP | pH 9.0, 25°C | Escherichia coli | |
| 0.09 | - |
dTTP | pH 9.0, 25°C | Escherichia coli | |
| 0.09 | - |
dCTP | dCTP in form of MgdCTP2- | Escherichia coli | |
| 0.09 | - |
dTTP | dTTP in form of MgdTTP2- | Escherichia coli | |
| 0.404 | - |
ATP | pH 9.0, 25°C | Escherichia coli | |
| 0.404 | - |
ATP | ATP in form of MgATP2- | Escherichia coli | |
| 2.008 | - |
CTP | pH 9.0, 25°C | Escherichia coli | |
| 2.008 | - |
CTP | CTP in form of MgCTP2- | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31500 | - |
isoform II, SDS-PAGE | Escherichia coli |
| 32500 | - |
isoform I, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 related isoforms | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.134 | - |
isoform I | Escherichia coli |
| 0.274 | - |
isoform II | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + tobramycin | - |
Escherichia coli | diphosphate + 2''-adenylyltobramycin | - |
r | |
| CTP + tobramycin | - |
Escherichia coli | diphosphate + 2''-cytosylyltobramycin | - |
r | |
| dATP + tobramycin | - |
Escherichia coli | diphosphate + 2''-deoxyadenylyltobramycin | - |
r | |
| dCTP + tobramycin | - |
Escherichia coli | diphosphate + 2''-deoxycytosylyltobramycin | - |
r | |
| dGTP + tobramycin | - |
Escherichia coli | diphosphate + 2''-deoxyguanosylyltobramycin | - |
r | |
| dTTP + tobramycin | - |
Escherichia coli | diphosphate + 2''-deoxythymidylyltobramycin | - |
r | |
| GTP + tobramycin | - |
Escherichia coli | diphosphate + 2''-guanosylyltobramycin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32500, isoform I, SDS-PAGE | Escherichia coli |
| monomer | 1 * 31500, isoform II, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAD 2'' | - |
Escherichia coli |
| ANT2'' | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
isoform I | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | - |
Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
MgGTP2- | pH 9.0, 25°C | Escherichia coli | |
| 0.011 | - |
MgdATP2- | pH 9.0, 25°C | Escherichia coli | |
| 0.027 | - |
MgdGTP | pH 9.0, 25°C | Escherichia coli | |
| 0.047 | - |
MgdCTP | pH 9.0, 25°C | Escherichia coli | |
| 0.145 | - |
MgdTTP | pH 9.0, 25°C | Escherichia coli | |
| 0.404 | - |
MgCTP | pH 9.0, 25°C | Escherichia coli | |
| 0.864 | - |
MgATP2- | pH 9.0, 25°C | Escherichia coli | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | 2 related isoforms, isoelectric focusing | - |
5.7 |
| Escherichia coli | 2 related isoforms, isoelectric focusing | - |
6 |
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