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Literature summary for 2.7.7.43 extracted from

  • Schaper, W.; Bentrop, J.; Ustinova, J.; Blume, L.; Kats, E.; Tiralongo, J.; Weinhold, B.; Bastmeyer, M.; Muenster-Kuehnel, A.K.
    Identification and biochemical characterization of two functional CMP-sialic acid synthetases in Danio rerio (2012), J. Biol. Chem., 287, 13239-13248.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.43

Cloned(Commentary)

Cloned (Comment) Organism
transfection of NIH-3T3 and EPC cells Danio rerio

Protein Variants

Protein Variants Comment Organism
additional information presence of four basic clusters BC1-BC4 in the protein sequence. Deletion of BC3 does not impair nuclear import of isoform Cmas1, deletion of BC1 or BC2, individually or in combination, entail retention in the cytoplasm. neither the deletion of BC1 nor BC2 alters enzymatic activity Danio rerio

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Danio rerio 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48200
-
 or pentamer, 4 * 48200, calculated Danio rerio
48200
-
 or tetramer, 5 * 48200, calculated Danio rerio
49600
-
 4 * 49600, calculated Danio rerio
193400
-
 gel filtration Danio rerio
236100
-
 gel filtration Danio rerio

Organism

Organism UniProt Comment Textmining
Danio rerio H9BFW7 isoform Cmas2
-
Danio rerio Q0E671 isoform Cmas1
-

Source Tissue

Source Tissue Comment Organism Textmining
brain during the pharyngula stage, expression is restricted to the brain Danio rerio
-
central nervous system robust expression in the entire central nervous system, the somites, the notochord, and the developing pronephric duct Danio rerio
-
gastrula more or less ubiquitously expressed from the end of gastrula through segmentation Danio rerio
-
additional information not detected in skeletal muscle, liver, or kidney Danio rerio
-
notochord robust expression in the entire central nervous system, the somites, the notochord, and the developing pronephric duct Danio rerio
-
somite robust expression in the entire central nervous system, the somites, the notochord, and the developing pronephric duct Danio rerio
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 substrate N-glycolylneuraminate, pH 7.5, temperature not specified in the publication Danio rerio
0.3
-
 substrate 5-deaminoneuraminate, pH 7.5, temperature not specified in the publication Danio rerio
2.7
-
 substrate N-glycolylneuraminate, pH 7.5, temperature not specified in the publication Danio rerio
3.3
-
 substrate 5-deaminoneuraminate, pH 7.5, temperature not specified in the publication Danio rerio
4.5
-
 substrate N-acetylneuraminate, pH 7.5, temperature not specified in the publication Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 5-deaminoneuraminate
-
 Danio rerio diphosphate + CMP-5-deaminoneuraminate
-
 ?
CTP + N-acetyl-neuraminate
-
 Danio rerio diphosphate + CMP-N-acetylneuraminate
-
 ?
CTP + N-glycolylneuraminate
-
 Danio rerio diphosphate + CMP-N-glycolylneuraminate
-
 ?
additional information enzyme is strictly dependent on CTP Danio rerio ?
-
 ?

Subunits

Subunits Comment Organism
pentamer or tetramer, 5 * 48200, calculated Danio rerio
tetramer 4 * 49600, calculated Danio rerio
tetramer or pentamer, 4 * 48200, calculated Danio rerio

Synonyms

Synonyms Comment Organism
Cmas1
-
 Danio rerio
Cmas2
-
 Danio rerio