Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Neisseria meningitidis |
expression in Escherichia coli | Pasteurella multocida |
expression in Escherichia coli | [Haemophilus] ducreyi |
Crystallization (Comment) | Organism |
---|---|
modeling of the closed conformation. Enzyme forms a dimer complex. Amino acids involved in the Neu5Ac binding pocket are Glu162 and Arg165 from monomer B and Gln104, Ser82, Val86, Tyr179, and Phe192 from monomer A | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
Q163A | mutant displays improved substrate promiscuity | Neisseria meningitidis |
S31R | mutant displays improved substrate promiscuity, catalytic activities for substrates N-glycolylneuraminate, N-methylglycolylneuraminate and 8-O-methyl N-acetyl-neuraminate are improved compared to wild-type | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
N-acetylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
0.14 | - |
N-acetylneuraminate | wild-type, pH 8.5, 37°C | Pasteurella multocida | |
0.16 | - |
CTP | wild-type, pH 8.5, 37°C | [Haemophilus] ducreyi | |
0.18 | - |
N-acetylneuraminate | wild-type, pH 8.5, 37°C | [Haemophilus] ducreyi | |
0.22 | - |
N-acetylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
0.22 | - |
CTP | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
0.24 | - |
CTP | wild-type, pH 8.5, 37°C | Pasteurella multocida | |
0.59 | - |
CTP | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
1.5 | - |
8-O-methyl-N-acetylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
2 | - |
N-methylglycolylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
4 | - |
N-glycolylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
5.3 | - |
8-O-methyl-N-acetylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
6.2 | - |
N-glycolylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
7 | - |
N-methylglycolylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
- |
- |
Pasteurella multocida | - |
- |
- |
Pasteurella multocida ATCC 15742 | - |
- |
- |
[Haemophilus] ducreyi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 8-O-methyl-N-acetylneuraminate | - |
Neisseria meningitidis | diphosphate + CMP-(8-O-methyl)-N-acetylneuraminate | - |
? | |
CTP + N-acetylneuraminate | - |
Neisseria meningitidis | diphosphate + CMP-N-acetylneuraminate | - |
? | |
CTP + N-acetylneuraminate | - |
Pasteurella multocida | diphosphate + CMP-N-acetylneuraminate | - |
? | |
CTP + N-acetylneuraminate | - |
[Haemophilus] ducreyi | diphosphate + CMP-N-acetylneuraminate | - |
? | |
CTP + N-acetylneuraminate | - |
Pasteurella multocida ATCC 15742 | diphosphate + CMP-N-acetylneuraminate | - |
? | |
CTP + N-glycolylneuraminate | - |
Neisseria meningitidis | diphosphate + CMP-N-glycolylneuraminate | - |
? | |
CTP + N-methylglycolylneuraminate | - |
Neisseria meningitidis | diphosphate + CMP-N-methylglycolylneuraminate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CSS | - |
Neisseria meningitidis |
CSS | - |
Pasteurella multocida |
CSS | - |
[Haemophilus] ducreyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
8-O-methyl-N-acetylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
2.9 | - |
N-methylglycolylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
3.2 | - |
N-glycolylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
4.2 | - |
N-acetylneuraminate | wild-type, pH 8.5, 37°C | Pasteurella multocida | |
4.6 | - |
CTP | wild-type, pH 8.5, 37°C | Pasteurella multocida | |
4.8 | - |
N-acetylneuraminate | wild-type, pH 8.5, 37°C | [Haemophilus] ducreyi | |
5.2 | - |
CTP | wild-type, pH 8.5, 37°C | [Haemophilus] ducreyi | |
7.4 | - |
N-acetylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
8.8 | - |
CTP | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
12 | - |
N-methylglycolylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
19 | - |
N-acetylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
21 | - |
CTP | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
21 | - |
8-O-methyl-N-acetylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
23 | - |
N-glycolylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Neisseria meningitidis |
8.5 | - |
- |
Pasteurella multocida |
8.5 | - |
- |
[Haemophilus] ducreyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Neisseria meningitidis |
7.5 | 9 | - |
Pasteurella multocida |
7.5 | 9 | - |
[Haemophilus] ducreyi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
8-O-methyl-N-acetylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
1.3 | - |
8-O-methyl-N-acetylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
1.6 | - |
N-methylglycolylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
1.7 | - |
N-methylglycolylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
3.8 | - |
N-glycolylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
5.9 | - |
N-glycolylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
19 | - |
CTP | wild-type, pH 8.5, 37°C | Pasteurella multocida | |
27 | - |
N-acetylneuraminate | wild-type, pH 8.5, 37°C | [Haemophilus] ducreyi | |
30 | - |
N-acetylneuraminate | wild-type, pH 8.5, 37°C | Pasteurella multocida | |
33 | - |
CTP | wild-type, pH 8.5, 37°C | [Haemophilus] ducreyi | |
36 | - |
CTP | wild-type, pH 8.5, 37°C | Neisseria meningitidis | |
40 | - |
CTP | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
74 | - |
N-acetylneuraminate | mutant S81R, pH 8.5, 37°C | Neisseria meningitidis | |
86 | - |
N-acetylneuraminate | wild-type, pH 8.5, 37°C | Neisseria meningitidis |