Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no enhancement of activity with dithiothreitol, glutathione, or beta-mercaptoethanol | Mannheimia haemolytica |
General Stability | Organism |
---|---|
the enzyme is equally stable with or without the presence of glycerol at different temperatures | Acetivibrio thermocellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-mercury-CTP | - |
Escherichia coli | |
EDTA | treatment with EDTA results in loss of catalytic activity which can be restored by the addition of either Mg2+ or Mn2+ | Escherichia coli | |
additional information | dithiothreitol has little effect on enzyme activity | [Haemophilus] ducreyi | |
p-hydroxymercuribenzoic acid | - |
Mannheimia haemolytica | |
periodate-oxidized CTP | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
CTP | at pH 7.1 and 37°C | [Haemophilus] ducreyi | |
0.13 | - |
N-acylneuraminate | - |
Acetivibrio thermocellus | |
0.24 | - |
CTP | - |
Acetivibrio thermocellus | |
0.26 | - |
N-acylneuraminate | at pH 7.1 and 37°C | [Haemophilus] ducreyi | |
0.31 | - |
CTP | - |
Neisseria meningitidis | |
0.31 | - |
CTP | at 37°C | Escherichia coli | |
0.34 | - |
N-acylneuraminate | - |
Neisseria meningitidis | |
1.77 | - |
CTP | - |
Mannheimia haemolytica | |
1.82 | - |
N-acylneuraminate | - |
Mannheimia haemolytica | |
4 | - |
N-acylneuraminate | at 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | partially substitutes Mg2+ in catalysis | Mannheimia haemolytica | |
Co2+ | partially substitutes Mg2+ in catalysis | Mannheimia haemolytica | |
Mg2+ | required for activity | Neisseria meningitidis | |
Mg2+ | absolutely required for activity, 20 mM Mg2+ gives the most activity | [Haemophilus] ducreyi | |
Mg2+ | required for activity, 20-40 mM Mg2+ is the optimum concentration | Escherichia coli | |
Mg2+ | required for activity, maximum activity at 10 mM Mg2+, decreasing at higher Mg2+ concentrations | Mannheimia haemolytica | |
Mn2+ | required for activity | Neisseria meningitidis | |
Mn2+ | partially substitutes Mg2+ in catalysis | Mannheimia haemolytica | |
Mn2+ | required for activity, can be replaced by Mg2+, Co2+, and Zn2+ in decreasing order | Acetivibrio thermocellus | |
Mn2+ | required for activity, Mn2+ is only 40% as effective as Mg2+ at 20 mM | [Haemophilus] ducreyi | |
additional information | Ca2+ shows no effect on enzyme activity | [Haemophilus] ducreyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24800 | - |
gel filtration | Neisseria meningitidis |
25400 | - |
gel filtration | [Haemophilus] ducreyi |
26000 | - |
SDS-PAGE | Acetivibrio thermocellus |
43000 | - |
SDS-PAGE | Mannheimia haemolytica |
49000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | Escherichia coli | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | Neisseria meningitidis | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | Mannheimia haemolytica | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | Acetivibrio thermocellus | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | [Haemophilus] ducreyi | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | Escherichia coli K-235 | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | Mannheimia haemolytica A2 | - |
diphosphate + CMP-N-acylneuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Escherichia coli | - |
- |
- |
Escherichia coli K-235 | - |
- |
- |
Mannheimia haemolytica | - |
- |
- |
Mannheimia haemolytica A2 | - |
- |
- |
Neisseria meningitidis | - |
- |
- |
[Haemophilus] ducreyi | - |
- |
- |
Storage Stability | Organism |
---|---|
-20°C or -80°C, in 50% (v/v) glycerol, several months, remains stable | [Haemophilus] ducreyi |
4°C, at pH 7.0, several weeks, remains stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + N-acetylneuraminate | - |
Mannheimia haemolytica | phosphate + CMP-N-acetylneuraminate | - |
? | |
CDP + N-acetylneuraminate | - |
Mannheimia haemolytica A2 | phosphate + CMP-N-acetylneuraminate | - |
? | |
CTP + (N-4-O-)diacetylneuraminic acid | (N-4-O-)diacetylneuraminic acid is 46% effective compared to N-acylneuraminate | Neisseria meningitidis | diphosphate + CMP-N-4-O-diacetylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Escherichia coli | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Neisseria meningitidis | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Mannheimia haemolytica | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Acetivibrio thermocellus | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
[Haemophilus] ducreyi | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Escherichia coli K-235 | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Mannheimia haemolytica A2 | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
CTP + N-glycolylneuraminic acid | - |
Acetivibrio thermocellus | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
CTP + N-glycolylneuraminic acid | - |
[Haemophilus] ducreyi | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
CTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica A2 | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
additional information | does not accept N-glycolylneuraminic acid and triacetylneuraminic acid as substrates | Neisseria meningitidis | ? | - |
? | |
additional information | does not utilize N-glycolylneuraminic acid as a substrate | Escherichia coli | ? | - |
? | |
additional information | does not utilize N-glycolylneuraminic acid as a substrate | Escherichia coli K-235 | ? | - |
? | |
TTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica | TMP-N-glycolylneuraminate + diphosphate | - |
? | |
TTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica A2 | TMP-N-glycolylneuraminate + diphosphate | - |
? | |
UDP + N-acylneuraminate | - |
Mannheimia haemolytica | phosphate + UMP-N-acetylneuraminate | - |
? | |
UDP + N-acylneuraminate | - |
Mannheimia haemolytica A2 | phosphate + UMP-N-acetylneuraminate | - |
? | |
UTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica | UMP-N-glycolylneuraminate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CMP-N-acetylneuraminic acid synthetase | - |
Escherichia coli |
CMP-N-acetylneuraminic acid synthetase | - |
Neisseria meningitidis |
CMP-N-acetylneuraminic acid synthetase | - |
Mannheimia haemolytica |
CMP-N-acetylneuraminic acid synthetase | - |
Acetivibrio thermocellus |
CMP-N-acetylneuraminic acid synthetase | - |
[Haemophilus] ducreyi |
CSS | - |
Escherichia coli |
CSS | - |
Neisseria meningitidis |
CSS | - |
Mannheimia haemolytica |
CSS | - |
Acetivibrio thermocellus |
CSS | - |
[Haemophilus] ducreyi |
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
Escherichia coli |
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
Neisseria meningitidis |
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
Mannheimia haemolytica |
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
Acetivibrio thermocellus |
cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
[Haemophilus] ducreyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Escherichia coli |
37 | - |
- |
Neisseria meningitidis |
37 | - |
- |
[Haemophilus] ducreyi |
50 | - |
- |
Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | the enzyme loses complete activity after 50 and 10 min when stored at 40°C and 45°C, respectively | Escherichia coli |
70 | - |
the enzyme retains activity at 70°C | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | - |
[Haemophilus] ducreyi |
8.5 | - |
- |
Neisseria meningitidis |
9 | - |
- |
Mannheimia haemolytica |
9 | 10 | - |
Escherichia coli |
9.5 | - |
- |
Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 13 | - |
Acetivibrio thermocellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 12.5 | activity is drastically reduced below pH 7.0 and above pH 12.5 | Mannheimia haemolytica |