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Literature summary for 2.7.7.43 extracted from

  • Vann, W.F.; Silver, R.P.; Abeijon, C.; Chang, K.; Aaronson, W.; Sutton, A.; Finn, C.W.; Lindner, W.; Kotsatos, M.
    Purification, properties, and genetic location of Escherichia coli cytidine 5-monophosphate N-acetylneuraminic acid synthetase (1987), J. Biol. Chem., 262, 17556-17562.
    View publication on PubMed
Search for more literature for 2.7.7.43

Inhibitors

Inhibitors Comment Organism Structure
5-Mercuri-CTP
-
 Escherichia coli
EDTA
-
 Escherichia coli
periodate-oxidized CTP
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 CTP pH 9.0, 37°C Escherichia coli
4
-
 N-acetylneuraminate pH 9.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ or Mn2+ required, optimal activity with 20-40 mM Mg2+ Escherichia coli
Mn2+ Mg2+ or Mn2+ required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 O18:K1
-
Escherichia coli O18:K1
-
 O18:K1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
4°C, above pH 7.0, stable for several weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP + N-acetylneuraminate
-
 Escherichia coli phosphate + CMP-N-acetylneuraminate
-
 ?
CDP + N-acetylneuraminate
-
 Escherichia coli O18:K1 phosphate + CMP-N-acetylneuraminate
-
 ?
CTP + N-acylneuraminate
-
 Escherichia coli diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-acylneuraminate
-
 Escherichia coli O18:K1 diphosphate + CMP-N-acylneuraminate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 above, 4°C, stable for 4 weeks Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
 5