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Literature summary for 2.7.7.43 extracted from

  • Kean, E.L.; Roseman, S.
    The sialic acids. X. Purification and properties of cytidine 5-monophosphosialic acid synthetase (1966), J. Biol. Chem., 241, 5643-5650.
    View publication on PubMed
Search for more literature for 2.7.7.43

General Stability

General Stability Organism
not stable to freezing and thawing, addition of substrates partially stabilizes the purified enzyme to freezing and thawing Sus scrofa
not stable to lyophilization Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
Cd2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
Co2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
Cu2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
Mn2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
Zn2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 CTP pH 9.0, 37°C Sus scrofa
0.8
-
 N-acylneuraminate pH 9.0, 37°C Sus scrofa
2.3
-
 N-glycolylneuraminate pH 9.0, 37°C Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required, at 20 mM 20% as effective as Mg2+ Sus scrofa
Co2+ divalent cation required, at 20 mM 10% as effective as Mg2+ Sus scrofa
Fe2+ divalent cation required, at 20 mM 10% as effective as Mg2+ Sus scrofa
Mg2+
-
 Homo sapiens
Mg2+ divalent cation required, maximal stimulation at 20 mM Sus scrofa
Mn2+ divalent cation required, at 20 mM 20% as effective as Mg2+ Sus scrofa

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Ovis aries
-
-
-
Sus scrofa
-
 hog
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Sus scrofa
-
epididymis
-
 Sus scrofa
-
kidney
-
 Sus scrofa
-
liver
-
 Sus scrofa
-
lung
-
 Sus scrofa
-
mammary gland
-
 Sus scrofa
-
spleen
-
 Sus scrofa
-
submaxillary gland
-
 Homo sapiens
-
submaxillary gland
-
 Sus scrofa
-
submaxillary gland
-
 Bos taurus
-
submaxillary gland
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.83
-
 at pH 7.6 Sus scrofa
1.58 3.17 at pH 9.0 Sus scrofa

Storage Stability

Storage Stability Organism
4°C, stable for 3 weeks Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + N-acylneuraminate
-
 Homo sapiens diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-acylneuraminate
-
 Sus scrofa diphosphate + CMP-N-acylneuraminate
-
 r
CTP + N-acylneuraminate
-
 Bos taurus diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-acylneuraminate
-
 Ovis aries diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-glycolylneuraminate
-
 Sus scrofa diphosphate + CMP-N-glycolylneuraminate
-
 r
CTP + N-glycolylneuraminate
-
 Bos taurus diphosphate + CMP-N-glycolylneuraminate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 not stable at room temperature for 12 h Sus scrofa
37
-
 reaction rate is proportional to enzyme concentration and constant for at least 45 min. At high enzyme concentrations the reaction rate decreases 10-15% between 45 and 60 min Sus scrofa