Activating Compound | Comment | Organism | Structure |
---|---|---|---|
PII proteins | PII proteins only stimulate the adenylylation activity of ATase | Rhodospirillum rubrum |
Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | sequences for the active sites for adenylylation and deadenylylation as in Escherichia coli are also present in Rhodospirillum rubrum, between amino acids 107-329 and 632-849, respectively, however a Q-linker as in Escherichia coli can not be identified | Rhodospirillum rubrum |
additional information | the C-terminal part of the protein between amino acids 1006-1149 shows high similarity to an Escherichia coli bacterioferritin comigratory protein (BCP) | Rhodospirillum rubrum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-ketoglutarate | in Rhodospirillum rubrum alpha-ketoglutarate inhibits the adenylylation activity of ATase | Rhodospirillum rubrum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K2HPO4 | deadenylylation assay with 10mM K2HPO4 | Rhodospirillum rubrum | |
Mg2+ | adenylylation assay with 10mM Mg2+ | Rhodospirillum rubrum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
126200 | - |
estimated from gene sequence | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutamine synthase + ATP | Rhodospirillum rubrum | ATase primarily regulated by alpha-ketoglutarate, glutamine has no effect on neither the adenylylation nor the deadenylylation of glutamine synthetase, PII proteins only stimulate the adenylylation reaction | adenylyl-[glutamine synthase] + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ATase is cloned into the pET101/D-TOPO plasmid, containing ATase fused to a C-terminal V5 epitope followed by six histidines, His-tagged proteins are applied to HisTrap HP 1ml column | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutamine synthase + ATP | ATase primarily regulated by alpha-ketoglutarate, glutamine has no effect on neither the adenylylation nor the deadenylylation of glutamine synthetase, PII proteins only stimulate the adenylylation reaction | Rhodospirillum rubrum | adenylyl-[glutamine synthase] + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
adenylyltransferase | - |
Rhodospirillum rubrum |
ATASE | - |
Rhodospirillum rubrum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Rhodospirillum rubrum |