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Literature summary for 2.7.7.41 extracted from
- Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria (2013), Cell Metab., 17, 709-718.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D220A | site-directed mutagenesis of TAM41, catalytically inactive mutant | Saccharomyces cerevisiae |
| Y130A | site-directed mutagenesis of TAM41, catalytically inactive mutant | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
1-palmitoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-glycero-3-phosphate | pH 7.5, 30°C | Saccharomyces cerevisiae | |
| 0.067 | - |
phosphatidate | NBD-phosphatidate, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.076 | - |
CTP | with NBD-phosphatidate, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.76 | - |
CTP | pH 7.5, 30°C | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | isoform Cds1 is an ER-resident protein | Saccharomyces cerevisiae | 5783 | - |
| mitochondrial inner membrane | - |
Saccharomyces cerevisiae | 5743 | - |
| mitochondrion | isoform Tam41 directly catalyzes the formation of CDP-diacylglycerol from phosphatidic acid in the mitochondrial inner membrane | Saccharomyces cerevisiae | 5739 | - |
| additional information | subcellular localization analysis of CDP-diacylglycerol synthases in yeast | Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | about 80% of the activity with Mg2+ | Saccharomyces cerevisiae | |
| Cu2+ | about 30% of the activity with Mg2+ | Saccharomyces cerevisiae | |
| Mg2+ | required | Saccharomyces cerevisiae | |
| Mg2+ | required fro activity | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + phosphatidate | Saccharomyces cerevisiae | - |
diphosphate + CDP-diacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P38221 | - |
- |
| Saccharomyces cerevisiae | P53230 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 1-palmitoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-glycero-3-phosphate | i.e. commercial substrate NBD-PA | Saccharomyces cerevisiae | diphosphate + CDP-1-palmitoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]glycerol | - |
? | |
| CTP + phosphatidate | - |
Saccharomyces cerevisiae | diphosphate + CDP-diacylglycerol | - |
? | |
| CTP + phosphatidate | NBD-phosphatidate | Saccharomyces cerevisiae | diphosphate + CDP-diacylglycerol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDP-DAG synthase | - |
Saccharomyces cerevisiae |
| CDP-diacylglycerol synthase | - |
Saccharomyces cerevisiae |
| CDS1 | - |
Saccharomyces cerevisiae |
| Tam41 | - |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | both CDP-diacylglycerol synthases Tam41 and Cds1 are highly conserved proteins, yet their origins seem to be different. The N-terminal portion of Tam41 possesses the NTase (Nucleotide Transferase) fold, which is consistent with the CDP-DAG synthase function of Tam41. In contrast, Cds1 exhibits the CDP-DAG synthase i.e. NTase activity, yet it does not contain the NTase fold. Cds1 and Tam41 have developed their own commitment to lipid biosynthetic pathways operating in two evolutionary distinct organelles, the endoplasmic reticulum and mitochondria, during evolution of eukaryotic cells | Saccharomyces cerevisiae |
| malfunction | knockout mutant DELTAtam41 cells show severe growth defects on fermentable or non-fermentable media at high temperature, steady-state levels of cytochrome c1 of complex III and cytochrome oxidase subunit IV (Cox4) of complex IV are decreased in the mutant cells. Overexpression of Art5 (a arrestin-related trafficking adaptor family member) restores the temperature-sensitive growth defects of DELTAtam41 cells completely on fermentable media but only partly on non-fermentable media, which renders the cell growth dependent on mitochondrial respiration. Overexpression of both Cds1 and Art5 rescues growth defects of pgs1DELTAtam41DELTA cells. The growth defects of DELTAtam41 at elevated temperature are suppressed by the simultaneous deletion of the ITR1 gene | Saccharomyces cerevisiae |
| metabolism | CDP-diacylglycerol synthase Cds1 resides in the endoplasmic reticulum but not in mitochondria, while CDP-diacylglycerol synthase Tam41, a highly conserved mitochondrial maintenance protein, directly catalyzes the formation of CDP-DAG from phosphatidate in the mitochondrial inner membrane | Saccharomyces cerevisiae |
| physiological function | inositol depletion by overexpressing an arrestin-related protein Art5 partially restores the defects of cell growth and cardiolipin synthesis in the absence of Tam41 | Saccharomyces cerevisiae |
| physiological function | the CDP-diacylglycerol synthase Tam41 is required for cardiolipin biosynthesis in mitochondria | Saccharomyces cerevisiae |
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