Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | a maximal stimulation of about 3fold with CTP as substrate and 1.25fold with dCTP | Escherichia coli |
General Stability | Organism |
---|---|
solubilized enzyme is stable to freezing and thawing | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CDP-dipalmitin | inhibits diphosphorolysis of CDP-dipalmitin | Escherichia coli | |
CTP | competitive with dCTP | Escherichia coli | |
dCTP | competitive with respect to CTP | Escherichia coli | |
EDTA | - |
Escherichia coli | |
SDS | 0.1% w/v | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
phosphatidic acid | pH 7, 37°C | Escherichia coli | |
0.9 | - |
dCTP | pH 7, 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell envelope | - |
Escherichia coli | 30313 | - |
membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | Escherichia coli | the enzyme produces key intermediates in phospholipid biosynthesis | diphosphate + CDPdiacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + phosphatidate = diphosphate + CDP-diacylglycerol | sequential mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.028 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, solubilized enzyme is stable for at least several months | Escherichia coli |
0-4°C, solubilized enzyme is stable for at least several weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | - |
Escherichia coli | diphosphate + CDPdiacylglycerol | - |
r | |
CTP + phosphatidate | the enzyme produces key intermediates in phospholipid biosynthesis | Escherichia coli | diphosphate + CDPdiacylglycerol | - |
? | |
dCTP + phosphatidate | - |
Escherichia coli | diphosphate + dCDPdiacylglycerol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
30 min, no loss of activity of membrane-bound enzyme, 70% loss of activity of partially purified enzyme | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
stability is good at pH 7 and dropps rapidly above | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
CTP | pH 7, 37°C | Escherichia coli | |
0.75 | - |
dCTP | pH 7, 37°C | Escherichia coli |