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Literature summary for 2.7.7.41 extracted from
- Purification of CDPdiacylglycerol synthase from Escherichia coli (1992), Methods Enzymol., 209, 237-242.
General Stability
| General Stability | Organism |
|---|---|
| the pure enzyme can be dialyzed, but to maintain activity the dialyzing buffer must contain 1% n-octyl-beta-D-glucopyranoside, 0.25 mM dithiothreitol and at least 200 mM salt | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
phosphatidic acid | - |
Escherichia coli |  |
| 0.58 | - |
dCTP | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, SDS-PAGE | Escherichia coli |
| 27570 | - |
x * 27570, calculation from gene sequence | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + phosphatidate | Escherichia coli | the activity is essential for all phospholipid biosynthesis in Escherichia coli | diphosphate + CDPdiacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.79 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified enzyme can be stored frozen for months if quick-frozen in liquid nitrogen | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + phosphatidate | the enzyme is specific for long-chain phosphatidic acid. 1-Acyl-sn-glycero-3-phosphate is not a substrate and phosphatidic acids with acyl chains shorter than 16 carbons are poor substrates | Escherichia coli | diphosphate + CDPdiacylglycerol | - |
? | |
| CTP + phosphatidate | the activity is essential for all phospholipid biosynthesis in Escherichia coli | Escherichia coli | diphosphate + CDPdiacylglycerol | - |
? | |
| dCTP + phosphatidate | - |
Escherichia coli | diphosphate + dCDPdiacylglycerol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, SDS-PAGE | Escherichia coli |
| ? | x * 27570, calculation from gene sequence | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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