Cloned (Comment) | Organism |
---|---|
overexpression of TarI and TarJ individually from their respective genes using the T7 expression system | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CDP-ribitol | competitive versus CTP | Haemophilus influenzae | |
CDP-ribitol | competitive versus CTP | Staphylococcus aureus | |
diphosphate | - |
Haemophilus influenzae | |
diphosphate | - |
Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00851 | - |
CTP | pH 7.2 | Haemophilus influenzae | |
0.0147 | - |
D-ribitol 5-phosphate | pH 7.2 | Haemophilus influenzae | |
0.0789 | - |
CTP | pH 7.2 | Staphylococcus aureus | |
1.28 | - |
D-ribitol 5-phosphate | pH 7.2 | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000 + 2 * 41000, TarI2J2, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE | Staphylococcus aureus |
41000 | - |
2 * 26000 + 2 * 41000, TarI2J2, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE | Staphylococcus aureus |
53190 | - |
2 * 53190, calculation from amino acid composition | Haemophilus influenzae |
106400 | - |
amino acid composition | Haemophilus influenzae |
129000 | - |
calculation from gel filtration and sedimentation velocity data | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + D-ribitol 5-phosphate | ordered bi-bi mechanism | Haemophilus influenzae | diphosphate + CDP-ribitol | - |
? | |
CTP + D-ribitol 5-phosphate | tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex. Release and free diffusion of the intermediate ribitol 5-phosphate without channelling between active sites, ordered bi-bi mechanism | Staphylococcus aureus | diphosphate + CDP-ribitol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 2 * 26000 + 2 * 41000, TarI2J2, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE | Staphylococcus aureus |
? | 2 * 53190, calculation from amino acid composition | Haemophilus influenzae |
Synonyms | Comment | Organism |
---|---|---|
Bcs1 | - |
Haemophilus influenzae |
cytidylyltransferase | - |
Staphylococcus aureus |
cytidylyltransferase | - |
Haemophilus influenzae |
TarIJ | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
CTP | pH 7.2 | Staphylococcus aureus | |
23.5 | - |
CTP | pH 7.2 | Haemophilus influenzae | |
76.8 | - |
CTP | pH 7.2 | Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki(intercept) and Ki(slope)-values | Staphylococcus aureus | |
additional information | - |
additional information | Ki(intercept) and Ki(slope)-values | Haemophilus influenzae |