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Literature summary for 2.7.7.4 extracted from
- Dissimilatory ATP sulfurylase from the hyperthermophilic sulfate reducer Archaeoglobus fulgidus belongs to the group of homo-oligomeric ATP sulfurylases (1998), FEMS Microbiol. Lett., 162, 257-264.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Eacherichia coli | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
diphosphate | - |
Archaeoglobus fulgidus |  |
| 0.17 | - |
adenosine 5'-phosphosulfate | - |
Archaeoglobus fulgidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, SDS-PAGE | Archaeoglobus fulgidus |
| 150000 | - |
gel filtration | Archaeoglobus fulgidus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | Archaeoglobus fulgidus | constitutive enzyme | diphosphate + adenylylsulfate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
DSM 4304T | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Archaeoglobus fulgidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.1 | - |
cell extract | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | - |
Archaeoglobus fulgidus | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | constitutive enzyme | Archaeoglobus fulgidus | diphosphate + adenylylsulfate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
wild-type enzyme | Archaeoglobus fulgidus |
| 100 | - |
recombinant enzyme | Archaeoglobus fulgidus |
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