Any feedback?
Literature summary for 2.7.7.4 extracted from
- Purification, properties and substrate specificity of adenosine triphosphate sulphurylase from spinach leaf tissue (1972), Biochem. J., 127, 237-247.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
ATP | pH 7.8, 35°C | Spinacia oleracea |  |
| 0.84 | - |
dATP | pH 7.8, 35°C | Spinacia oleracea | |
| 1 | - |
SeO42- | pH 7.8, 35°C | Spinacia oleracea | |
| 3.1 | - |
SO42- | pH 7.8, 35°C | Spinacia oleracea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Mg2+ or Co2+ required | Spinacia oleracea | |
| Mg2+ | Mg2+ or Co2+ required | Spinacia oleracea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, stable for at least 4 months | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + SeO42- | - |
Spinacia oleracea | AMP + adenylylselenate | - |
? | |
| ATP + sulfate | - |
Spinacia oleracea | diphosphate + adenylylsulfate | - |
r | |
| dATP + SO42- | - |
Spinacia oleracea | diphosphate + deoxyadenylylsulfate | - |
? | |
| additional information | sulfate is the only form of sulfur that catalyzes diphosphate-ATP exchange. The enzyme catalyzes diphosphate-dATP exchange. Selenate catalyzes diphosphate-ATP exchange, but no AMP is formed. Molybdate does not catalyze diphosphate-ATP exchange but AMP is formed | Spinacia oleracea | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
1 min, complete loss of activity | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | - |
Spinacia oleracea |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
