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Literature summary for 2.7.7.4 extracted from
- Sulfate activation in Rhodobacter sulfidophilus (1985), Arch. Microbiol., 141, 384-391.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3'-phosphoadenosine-5'-phosphate | - |
Rhodovulum sulfidophilum |  |
| ADP | - |
Rhodovulum sulfidophilum | |
| AMP | - |
Rhodovulum sulfidophilum | |
| S2O32- | - |
Rhodovulum sulfidophilum | |
| Sulfide | - |
Rhodovulum sulfidophilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
ATP | pH 8.0, 30°C | Rhodovulum sulfidophilum | |
| 0.33 | - |
SO42- | pH 8.0, 30°C | Rhodovulum sulfidophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 290000 | - |
gel filtration | Rhodovulum sulfidophilum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | Rhodovulum sulfidophilum | key enzyme of sulfate assimilation | diphosphate + adenylylsulfate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Blastochloris viridis | - |
- |
- |
| Cereibacter sphaeroides | - |
- |
- |
| Rhodobacter capsulatus | - |
- |
- |
| Rhodopseudomonas palustris | - |
- |
- |
| Rhodospirillum rubrum | - |
- |
- |
| Rhodovulum sulfidophilum | - |
- |
- |
| Rubrivivax gelatinosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodovulum sulfidophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.47 | - |
- |
Rhodovulum sulfidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | - |
Cereibacter sphaeroides | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Rubrivivax gelatinosus | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Rhodopseudomonas palustris | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Rhodobacter capsulatus | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Rhodospirillum rubrum | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Blastochloris viridis | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Rhodovulum sulfidophilum | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | key enzyme of sulfate assimilation | Rhodovulum sulfidophilum | diphosphate + adenylylsulfate | - |
r | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | full activity up to 40°C, completely inactive at 55°C | Rhodovulum sulfidophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rhodovulum sulfidophilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 9.4 | the enzyme is active in Tris-buffer between pH 6.8 and 9.4 | Rhodovulum sulfidophilum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
3'-phosphoadenosine-5'-phosphate | pH 8.0, 30°C | Rhodovulum sulfidophilum | |
| 0.4 | - |
S2O32- | pH 8.0, 30°C | Rhodovulum sulfidophilum | |
| 0.66 | - |
Sulfide | pH 8.0, 30°C | Rhodovulum sulfidophilum | |
| 1.15 | - |
ADP | pH 8.0, 30°C | Rhodovulum sulfidophilum | |
| 2.1 | - |
AMP | pH 8.0, 30°C | Rhodovulum sulfidophilum | |
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