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Literature summary for 2.7.7.4 extracted from
- Co-purification and characterization of ATP-sulfurylase and adenosine-5-phosphosulfate kinase from rat chondrosarcoma (1987), J. Biol. Chem., 262, 7374-7382.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00162 | - |
adenosine 5'-phosphosulfate | - |
Rattus norvegicus | |
| 0.0389 | - |
diphosphate | pH 7.8, 37°C | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 50-60% of the activity with Mg2+ | Rattus norvegicus | |
| Co2+ | 50-60% of the activity with Mg2+ | Rattus norvegicus | |
| Mg2+ | Mg2+ is the most efficient divalent cation, Km: 0.122 mM | Rattus norvegicus | |
| Mn2+ | 84% of the activity with Mg2+ | Rattus norvegicus | |
| Zn2+ | 50-60% of the activity with Mg2+ | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
non-denaturing PAGE | Rattus norvegicus |
| 260000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| chondrosarcoma cell | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.953 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sulfate | - |
Rattus norvegicus | diphosphate + adenylylsulfate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000-37000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | - |
bimodal temperature optima: 46°C and 52-54°C | Rattus norvegicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 60 | 35°C: about 40% of maximal activity, 60°C: about 35% of maximal activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.4 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.4 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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