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Literature summary for 2.7.7.4 extracted from
- Regulation of inorganic sulfate activation in filamentous fungi. Allosteric inhibition of ATP sulfurylase by 3-phosphoadenosine-5-phosphosulfate (1990), J. Biol. Chem., 265, 10300-10308.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FSO3- | activates in presence of 0.15 mM of 3'-phosphoadenosine-5'-phosphate | Penicillium chrysogenum |  |
| S2O32- | activates SO42dependent reaction in presence of 0.15 mM of 3'-phosphoadenosine-5'-phosphate | Penicillium chrysogenum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3'-phosphoadenosine-5'-phosphate | strong | Aspergillus nidulans | |
| 3'-phosphoadenosine-5'-phosphate | strong | Neurospora crassa | |
| 3'-phosphoadenosine-5'-phosphate | strong | Penicillium chrysogenum | |
| 3'-phosphoadenosine-5'-phosphate | strong | Penicillium duponti | |
| 3'-phosphoadenosine-5'-phosphate | - |
Spinacia oleracea | |
| 3'-phosphoadenosine-5'-phosphosulfate | - |
Penicillium chrysogenum | |
| 3'-phosphoadenosine-5'-phosphosulfate | - |
Spinacia oleracea | |
| FSO3- | inhibition in absence of 3'-phosphoadenosine-5'-phosphate | Penicillium chrysogenum | |
| FSO3- | - |
Penicillium duponti | |
| FSO3- | - |
Rattus norvegicus | |
| MgATP2- | - |
Penicillium chrysogenum | |
| MoO42- | - |
Penicillium chrysogenum | |
| S2O32- | 1 mM, 62% inhibition | Penicillium chrysogenum | |
| S2O32- | - |
Penicillium duponti | |
| S2O32- | - |
Rattus norvegicus | |
| SO32- | 1 mM, 10-25% inhibition | Penicillium chrysogenum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
ATP | pH 8.0, 30°C, reaction with MoO42- | Penicillium chrysogenum | |
| 0.023 | - |
ATP | ATP in form of MgATP2- | Penicillium chrysogenum | |
| 0.093 | - |
MoO42- | pH 8.0, 30°C | Penicillium chrysogenum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, SDS-PAGE | Spinacia oleracea |
| 60000 | - |
x * 60000, SDS-PAGE | Saccharomyces cerevisiae |
| 62000 | - |
x * 62000, SDS-PAGE | Rattus norvegicus |
| 63000 | - |
x * 63000, SDS-PAGE | Neurospora crassa |
| 65000 | - |
x * 65000, SDS-PAGE | Aspergillus nidulans |
| 66000 | - |
x * 66000, SDS-PAGE | Penicillium duponti |
| 67000 | - |
x * 67000, SDS-PAGE | Penicillium chrysogenum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
| Neurospora crassa | - |
- |
- |
| Penicillium chrysogenum | - |
- |
- |
| Penicillium duponti | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MoO42- | - |
Penicillium chrysogenum | AMP + adenylylmolybdate | - |
? | |
| ATP + sulfate | - |
Rattus norvegicus | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Saccharomyces cerevisiae | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Aspergillus nidulans | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Neurospora crassa | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Penicillium chrysogenum | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Spinacia oleracea | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Penicillium duponti | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Penicillium chrysogenum | diphosphate + adenylyl sulfate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Rattus norvegicus |
| ? | x * 48000, SDS-PAGE | Spinacia oleracea |
| ? | x * 60000, SDS-PAGE | Saccharomyces cerevisiae |
| ? | x * 66000, SDS-PAGE | Penicillium duponti |
| ? | x * 67000, SDS-PAGE | Penicillium chrysogenum |
| ? | x * 63000, SDS-PAGE | Neurospora crassa |
| ? | x * 65000, SDS-PAGE | Aspergillus nidulans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000057 | - |
3'-phosphoadenosine-5'-phosphosulfate | pH 8.0, 30°C, competitive with MgATP2-, at 0.1 mM MoO42- | Spinacia oleracea | |
| 0.000063 | - |
3'-phosphoadenosine-5'-phosphosulfate | pH 8.0, 30°C, at 0.1 mM MoO42- | Penicillium chrysogenum | |
| 0.00007 | - |
3'-phosphoadenosine-5'-phosphosulfate | pH 8.0, 30°C, competitive with MoO42-, at 0.05 mM MoO42- | Spinacia oleracea | |
| 0.00008 | - |
3'-phosphoadenosine-5'-phosphosulfate | pH 8.0, 30°C, at 0.05 mM MgATP2- | Penicillium chrysogenum | |
| 0.0004 | - |
3'-phosphoadenosine-5'-phosphosulfate | pH 8.0, 30°C, at 20 mM MoO42- | Penicillium chrysogenum | |
| 0.0004 | - |
3'-phosphoadenosine-5'-phosphosulfate | at 5 mM MgATP2- | Penicillium chrysogenum | |
| 0.5 | - |
MgATP2- | pH 8.0, 30°C | Penicillium chrysogenum | |
| 2.02 | - |
MoO42- | pH 8.0, 30°C | Penicillium chrysogenum | |
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