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Literature summary for 2.7.7.4 extracted from
- Comparative stability and catalytic and chemical properties of the sulfate-activating enzymes from Penicillium chrysogenum (mesophile) and Penicillium duponti (thermophile) (1985), J. Bacteriol., 164, 674-683.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| FSO3- | - |
Penicillium chrysogenum |  |
| FSO3- | - |
Penicillium duponti | |
| S2O32- | - |
Penicillium chrysogenum | |
| S2O32- | - |
Penicillium duponti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
adenosine 5'-phosphosulfate | pH 8.0, 30°C | Penicillium chrysogenum | |
| 0.0003 | - |
adenosine 5'-phosphosulfate | pH 8.0, 30°C | Penicillium duponti | |
| 0.0065 | - |
diphosphate | pH 8.0, 30°C | Penicillium chrysogenum | |
| 0.0083 | - |
diphosphate | pH 8.0, 30°C | Penicillium chrysogenum | |
| 0.03 | - |
ATP | pH 8.0, 30°C, at saturating concentrations of MoO42- | Penicillium duponti | |
| 0.03 | - |
ATP | ATP in form of MgATP2- | Penicillium duponti | |
| 0.05 | - |
ATP | pH 8.0, 30°C, at saturating concentrations of MoO42- | Penicillium chrysogenum | |
| 0.05 | - |
ATP | ATP in form of MgATP2- | Penicillium chrysogenum | |
| 0.08 | - |
MoO42- | pH 8.0, 30°C | Penicillium duponti | |
| 0.11 | - |
MoO42- | pH 8.0, 30°C | Penicillium chrysogenum | |
| 0.18 | - |
ATP | pH 8.0, 30°C, at saturating concentrations of SO42- | Penicillium chrysogenum | |
| 0.18 | - |
ATP | ATP in form of MgATP2- | Penicillium chrysogenum | |
| 0.19 | - |
ATP | pH 8.0, 30°C, at saturating concentrations of SO42- | Penicillium duponti | |
| 0.19 | - |
ATP | ATP in form of MgATP2- | Penicillium duponti | |
| 0.55 | - |
SO42- | pH 8.0, 30°C | Penicillium chrysogenum | |
| 0.55 | - |
SO42- | pH 8.0, 30°C | Penicillium duponti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
6 * 69000, SDS-PAGE | Penicillium chrysogenum |
| 440000 | - |
gel filtration | Penicillium chrysogenum |
| 440000 | - |
gel filtration | Penicillium duponti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
| Penicillium duponti | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + molybdate | - |
Penicillium chrysogenum | diphosphate + adenylyl molybdate | - |
r | |
| ATP + molybdate | - |
Penicillium duponti | diphosphate + adenylyl molybdate | - |
r | |
| ATP + MoO42- | - |
Penicillium chrysogenum | AMP + adenylylmolybdate | - |
? | |
| ATP + MoO42- | - |
Penicillium duponti | AMP + adenylylmolybdate | - |
? | |
| ATP + sulfate | - |
Penicillium chrysogenum | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Penicillium duponti | diphosphate + adenylylsulfate | - |
r | |
| ATP + sulfate | - |
Penicillium chrysogenum | diphosphate + adenylyl sulfate | - |
r | |
| ATP + sulfate | - |
Penicillium duponti | diphosphate + adenylyl sulfate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 69000, SDS-PAGE | Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
in a 20 min assay | Penicillium chrysogenum |
| 20 | - |
in a 20 min assay | Penicillium duponti |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
enzyme of Penicillium chrysogenum denaturates with a rate constant nearly 100fold greater than that of the Penicillium duponti enzyme | Penicillium chrysogenum |
| 65 | - |
enzyme of Penicillium chrysogenum denaturates with a rate constant nearly 100fold greater than that of the Penicillium duponti enzyme | Penicillium duponti |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
enzyme of Penicillium chrysogenum denaturates with a rate constant nearly 100fold greater than that of the Penicillium duponti enzyme | Penicillium chrysogenum |
| 4.4 | - |
enzyme of Penicillium chrysogenum denaturates with a rate constant nearly 100fold greater than that of the Penicillium duponti enzyme | Penicillium duponti |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0034 | - |
FSO3- | pH 8.0, 30°C | Penicillium chrysogenum | |
| 0.004 | - |
FSO3- | pH 8.0, 30°C | Penicillium duponti | |
| 0.36 | - |
S2O32- | pH 8.0, 30°C | Penicillium chrysogenum | |
| 0.4 | - |
S2O32- | pH 8.0, 30°C | Penicillium duponti | |
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