Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.39 extracted from

  • Park, Y.S.; Gee, P.; Sanker, S.; Schurter, E.J.; Zuiderweg, E.R.; Kent, C.
    Identification of functional conserved residues of CTP:glycerol-3-phosphate cytidylyltransferase. Role of histidines in the conserved HXGH in catalysis (1997), J. Biol. Chem., 272, 15161-15166.
    View publication on PubMed
Search for more literature for 2.7.7.39

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
C106A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
D11A mutant with low kcat value Bacillus subtilis
D11E mutant with low kcat value Bacillus subtilis
D38A mutant with high Km value Bacillus subtilis
D38E kcat similar to wild-type enzyme Bacillus subtilis
D66A mutant with low kcat value, defective in overall structure Bacillus subtilis
D66E mutant with low kcat value Bacillus subtilis
D87A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
D92A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
D94A mutant with high Km value Bacillus subtilis
D94E mutant mutant with low kcat value Bacillus subtilis
E115A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
E39A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
E67A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
H14A mutant with low kcat value, dramatic loss of activity Bacillus subtilis
H17A mutant with low kcat value, dramatic loss of activity Bacillus subtilis
H84A mutant with low kcat value Bacillus subtilis
K103A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
K19A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
K22A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
K25A mutant with kinetic constants similar to wild-type enzyme Bacillus subtilis
R113A mutant with low kcat value Bacillus subtilis
R113K mutant with low kcat value Bacillus subtilis
R55A mutant with low kcat value Bacillus subtilis
R55K mutant with low kcat value Bacillus subtilis
R63A mutant with low kcat value Bacillus subtilis
S118A mutant with low kcat value t Bacillus subtilis
T114A mutant with low kcat value Bacillus subtilis
T119A mutant with low kcat value Bacillus subtilis
W74A mutant with high Km value Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 glycerol 3-phosphate D66E mutant Bacillus subtilis
0.5
-
 CTP H84A mutant Bacillus subtilis
0.53
-
 glycerol 3-phosphate H84A mutant Bacillus subtilis
0.63
-
 CTP D66E mutant and D94E mutant Bacillus subtilis
0.72
-
 CTP R55A mutant Bacillus subtilis
0.73
-
 CTP D11E mutant Bacillus subtilis
0.79
-
 glycerol 3-phosphate R63A mutant Bacillus subtilis
0.85
-
 glycerol 3-phosphate E39A mutant, D11E mutant and D38E mutant Bacillus subtilis
0.89
-
 glycerol 3-phosphate S118A mutant Bacillus subtilis
1
-
 CTP D38E mutant Bacillus subtilis
1.01
-
 glycerol 3-phosphate D94E mutant Bacillus subtilis
1.02
-
 CTP D11A mutant and R113A mutant Bacillus subtilis
1.03
-
 CTP H14A mutant Bacillus subtilis
1.06
-
 glycerol 3-phosphate K25A mutant Bacillus subtilis
1.07
-
 CTP R63A mutant Bacillus subtilis
1.09
-
 glycerol 3-phosphate wild-type Bacillus subtilis
1.14
-
 CTP S118A mutant Bacillus subtilis
1.19
-
 glycerol 3-phosphate C106A mutant Bacillus subtilis
1.25
-
 CTP E39A mutant Bacillus subtilis
1.26
-
 glycerol 3-phosphate H14A mutant Bacillus subtilis
1.27
-
 CTP H17A mutant Bacillus subtilis
1.3
-
 glycerol 3-phosphate T114A mutant Bacillus subtilis
1.33
-
 CTP C106A mutant Bacillus subtilis
1.37
-
 glycerol 3-phosphate K22A mutant Bacillus subtilis
1.39
-
 CTP wild-type Bacillus subtilis
1.4
-
 CTP R113K mutant Bacillus subtilis
1.44
-
 CTP K25A mutant Bacillus subtilis
1.46
-
 glycerol 3-phosphate K103A mutant Bacillus subtilis
1.56
-
 glycerol 3-phosphate T119A mutant Bacillus subtilis
1.62
-
 CTP T114A mutant Bacillus subtilis
1.64
-
 CTP T119A mutant Bacillus subtilis
1.72
-
 glycerol 3-phosphate D87A mutant and R55A mutant Bacillus subtilis
1.74
-
 CTP K103A mutant Bacillus subtilis
1.76
-
 glycerol 3-phosphate D11A mutant Bacillus subtilis
1.78
-
 glycerol 3-phosphate K19A mutant Bacillus subtilis
1.8
-
 CTP K22A mutant Bacillus subtilis
1.85
-
 glycerol 3-phosphate D92A mutant Bacillus subtilis
2
-
 glycerol 3-phosphate E67A mutant Bacillus subtilis
2.02
-
 CTP D66A mutant Bacillus subtilis
2.19
-
 CTP K19A mutant Bacillus subtilis
2.22
-
 glycerol 3-phosphate H17A mutant Bacillus subtilis
2.25
-
 CTP D92A mutant Bacillus subtilis
2.3
-
 CTP D87A mutant Bacillus subtilis
2.6
-
 glycerol 3-phosphate R113A mutant Bacillus subtilis
2.63
-
 CTP E67A mutant Bacillus subtilis
3.93
-
 glycerol 3-phosphate R113K mutant Bacillus subtilis
4.34
-
 glycerol 3-phosphate E115A mutant Bacillus subtilis
4.57
-
 CTP R55K mutant Bacillus subtilis
4.85
-
 glycerol 3-phosphate D66A mutant Bacillus subtilis
6.04
-
 CTP E115A mutant Bacillus subtilis
7.94
-
 CTP W74A mutant Bacillus subtilis
8.12
-
 glycerol 3-phosphate D38A mutant Bacillus subtilis
8.88
-
 CTP D38A mutant Bacillus subtilis
11.4
-
 glycerol 3-phosphate R55K mutant Bacillus subtilis
14.2
-
 glycerol 3-phosphate W74A mutant Bacillus subtilis
93.5
-
 CTP D94A mutant Bacillus subtilis
141
-
 glycerol 3-phosphate D94A mutant Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + sn-glycerol 3-phosphate Bacillus subtilis
-
 diphosphate + CDPglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + sn-glycerol 3-phosphate
-
 Bacillus subtilis diphosphate + CDPglycerol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 CTP H14A mutant Bacillus subtilis
0.007
-
 CTP H17A mutant and H84A mutant Bacillus subtilis
0.01
-
 CTP D66A mutant Bacillus subtilis
0.01
-
 CTP R113K mutant Bacillus subtilis
0.02
-
 CTP D11E mutant and D94E mutant Bacillus subtilis
0.03
-
 CTP D11A mutant Bacillus subtilis
0.05
-
 CTP R55A mutant Bacillus subtilis
0.333
-
 CTP R113A mutant Bacillus subtilis
1.23
-
 CTP S118A mutant Bacillus subtilis
1.45
-
 CTP T119A mutant Bacillus subtilis
1.55
-
 CTP D38A mutant Bacillus subtilis
1.63
-
 CTP T114A mutant Bacillus subtilis
2.7
-
 CTP R63A mutant Bacillus subtilis
3.05
-
 CTP D66E mutant Bacillus subtilis
3.42
-
 CTP D94A mutant Bacillus subtilis
4.32
-
 CTP R55K mutant Bacillus subtilis
9.37
-
 CTP W74A mutant Bacillus subtilis
12.3
-
 CTP E39A mutant Bacillus subtilis
13.7
-
 CTP E67A mutant Bacillus subtilis
13.9
-
 CTP C106A mutant Bacillus subtilis
18.1
-
 CTP K25A mutant Bacillus subtilis
18.8
-
 CTP wild-type Bacillus subtilis
21.6
-
 CTP E115A mutant Bacillus subtilis
22.3
-
 CTP K103A mutant Bacillus subtilis
23.6
-
 CTP K22A mutant Bacillus subtilis
23.8
-
 CTP D87A mutant Bacillus subtilis
24.1
-
 CTP D38E mutant Bacillus subtilis
24.9
-
 CTP K19A mutant Bacillus subtilis
26.2
-
 CTP D92A mutant Bacillus subtilis