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Literature summary for 2.7.7.38 extracted from
- CMP-KDO synthetase: overproduction and application to the synthesis of CMP-KDO and analogs (1995), Bioorg. Med. Chem., 3, 313-320.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene amplified from Escherichia coli K-235 DNA cloned and overexpressed in recombinant Escherichia coli | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel filtration, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 3-deoxy-D-manno-octulosonate | Escherichia coli | - |
diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
strains K-235, ATCC 13207, Sure and wKDO-9 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.1 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 3,5-dideoxy-5-fluoro-beta-D-manno-2-octulopyranosonic acid | - |
Escherichia coli | diphosphate + CMP-3,5-dideoxy-5-fluoro-beta-D-manno-2-octulopyranosonic acid | - |
r | |
| CTP + 3-deoxy-D-manno-octulosonate | - |
Escherichia coli | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
| CTP + 5-deoxy-Kdo | - |
Escherichia coli | diphosphate + CMP-5-deoxy-3-deoxy-D-manno-octulosonate | - |
r | |
| CTP + 5-epi-Kdo | - |
Escherichia coli | diphosphate + CMP-5-epi-3-deoxy-D-manno-octulosonate | - |
r | |
| CTP + 5-fluoro-2-keto-3,5-dideoxyoctulosonate | - |
Escherichia coli | diphosphate + CMP-5-fluoro-2-keto-3,5-dideoxy octulosonate | - |
r | |
| additional information | L-KDN is no substrate | Escherichia coli | ? | - |
? | |
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