KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
CTP | pH 9.5, 30°C | Escherichia coli | |
0.39 | - |
3-deoxy-D-manno-octulosonate | pH 9.5, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | much less effective than Mg2+ | Escherichia coli | |
Mg2+ | requires 10 mM for maximal activity, other divalent cations including Mn2+ and Co2+ are much less effective | Escherichia coli | |
Mn2+ | much less effective than Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
molecular sieving | Escherichia coli |
36000 | - |
1 * 36000, SDS-PAGE | Escherichia coli |
44000 | - |
sucrose density gradient centrifugation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 3-deoxy-D-manno-octulosonate | Escherichia coli | - |
diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
CTP + 3-deoxy-D-manno-octulosonate | Escherichia coli B / ATCC 11303 | - |
diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-90°C, purified protein, 0.05 M potassium phosphate buffer, 0.5 mM dithiothreitol, no loss detected for up to 4 months | Escherichia coli |
4°C, partially purified enzyme, no loss of activity for up to 1 month | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 3-deoxy-D-manno-octulosonate | - |
Escherichia coli | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
CTP + 3-deoxy-D-manno-octulosonate | - |
Escherichia coli B / ATCC 11303 | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
dCTP + 3-deoxy-D-manno-octulosonate | reaction at pH 8.0 | Escherichia coli | diphosphate + dCMP-3-deoxy-D-manno-octulosonate | - |
r | |
dCTP + 3-deoxy-D-manno-octulosonate | reaction at pH 8.0 | Escherichia coli B / ATCC 11303 | diphosphate + dCMP-3-deoxy-D-manno-octulosonate | - |
r | |
additional information | dUTP is no nucleotide acceptor for 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate will not serve as substrate in place of 3-deoxy-D-manno-octulosonate | Escherichia coli | ? | - |
? | |
additional information | dUTP is no nucleotide acceptor for 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate will not serve as substrate in place of 3-deoxy-D-manno-octulosonate | Escherichia coli B / ATCC 11303 | ? | - |
? | |
UTP + 3-deoxy-D-manno-octulosonate | reaction at pH 9.5, approximately 50% the rate with CTP | Escherichia coli | diphosphate + UMP-3-deoxy-D-manno-octulosonate | - |
r | |
UTP + 3-deoxy-D-manno-octulosonate | reaction at pH 9.5, approximately 50% the rate with CTP | Escherichia coli B / ATCC 11303 | diphosphate + UMP-3-deoxy-D-manno-octulosonate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
4 |