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Literature summary for 2.7.7.37 extracted from
- Phosphorolysis of the pyrophosphate bond of sugar nucleotides II. Purification and properties of the enzyme (1965), J. Biol. Chem., 240, 2114-2121.
General Stability
| General Stability | Organism |
|---|---|
| ultrafiltration or ammonium sulfate precipitation results in heavy loss of activity | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-mannose 1-phosphate | 1.25 mM: 22% inhibition, 4.2 mM: 40% inhibition | Saccharomyces cerevisiae |  |
| Mg2+ | 17 mM: 32% inhibition | Saccharomyces cerevisiae | |
| phosphate | 4.5 mM: 55% inhibition | Saccharomyces cerevisiae | |
| Zn2+ | 5 mM: complete inhibition | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
phosphate | pH 8.0, 30°C | Saccharomyces cerevisiae | |
| 0.25 | - |
UDP-mannose | pH 8.0, 30°C | Saccharomyces cerevisiae | |
| 1.5 | - |
UDP-glucose | pH 8.0, 30°C | Saccharomyces cerevisiae | |
| 6.2 | - |
GDP-mannose | pH 8.0, 30°C | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| protoplast | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 0.1-1 mM of Mg2+ or 1 mM of Zn2+ has no effect | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NDP + aldose 1-phosphate | Saccharomyces cerevisiae | - |
NDP-aldose + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.178 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, concentrated enzyme fraction, stable for several months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-mannose + phosphate | 30% of activity compared to GDPmannose | Saccharomyces cerevisiae | ADP + D-mannose 1-phosphate | - |
ir | |
| dTDP-glucose + phosphate | 11% of activity compared to GDPmannose | Saccharomyces cerevisiae | dTDP + D-glucose 1-phosphate | - |
ir | |
| dTDP-mannose + phosphate | 157% of activity compared to GDPmannose | Saccharomyces cerevisiae | dTDP + D-mannose 1-phosphate | - |
ir | |
| GDP-galactose + phosphate | 3% of activity compared to GDPmannose | Saccharomyces cerevisiae | GDP + D-galactose 1-phosphate | - |
ir | |
| GDP-mannose + phosphate | - |
Saccharomyces cerevisiae | GDP + D-mannose 1-phosphate | - |
ir | |
| additional information | no activity with GDP-glucose, ADP-glucose, ADP-galactose, ADP-xylose, dADP-glucose, IDP-glucose | Saccharomyces cerevisiae | ? | - |
? | |
| NDP + aldose 1-phosphate | - |
Saccharomyces cerevisiae | NDP-aldose + phosphate | - |
ir | |
| UDP-acetylglucosamine + phosphate | 5.1% of activity compared to GDPmannose | Saccharomyces cerevisiae | UDP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
ir | |
| UDP-galactose + phosphate | 18% of activity compared to GDP-mannose | Saccharomyces cerevisiae | UDP + D-galactose 1-phosphate | - |
ir | |
| UDP-glucose + phosphate | 22% of activity compared to GDP-mannose | Saccharomyces cerevisiae | UDP + D-glucose 1-phosphate | - |
ir | |
| UDP-mannose + phosphate | 290% of activity compared to GDP-mannose | Saccharomyces cerevisiae | UDP + D-mannose 1-phosphate | - |
ir | |
| UDP-xylose + phosphate | 24.5% of activity compared to GDP-mannose | Saccharomyces cerevisiae | UDP + D-xylose 1-phosphate | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
15 min: 40% remaining activity, 30 min: 27% remaining activity | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | 14% of maximal activity at pH 6.5, 43% of maximal activity at pH 9.5 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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