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Literature summary for 2.7.7.33 extracted from

  • Rubenstein, P.A.; Strominger, J.L.
    Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexosis. IX. Purification and properties of the cytidine diphosphate-D-glucose pyrophosphorylase from Pasteurella pseudotuberculosis. type V (1974), J. Biol. Chem., 249, 3789-3796.
    View publication on PubMed
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General Stability

General Stability Organism
during the latter stages of purification the enzyme preparation is quite unstable to cold room conditions over a period of 2 to 3 days Yersinia pseudotuberculosis
Stable to repeated freeze-thawing Yersinia pseudotuberculosis

Inhibitors

Inhibitors Comment Organism Structure
CDP-4-keto-6-deoxy-D-glucose
-
 Yersinia pseudotuberculosis
CDP-ascarylose synonym: 3,6-dideoxy-L-mannose, strong, kinetics Yersinia pseudotuberculosis
CDP-D-fucose/CDP-6-deoxy-D-glucose mixture
-
 Yersinia pseudotuberculosis
CDPabequose synonym: 3,6-dideoxy-D-galactose, strong Yersinia pseudotuberculosis
additional information no inhibition by abequose Yersinia pseudotuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 CTP pH 7.5, 30°C Yersinia pseudotuberculosis
0.11
-
 alpha-D-glucose-1-phosphate pH 7.5, 30°C Yersinia pseudotuberculosis
0.41
-
 CDP-D-glucose pH 7.5, 30°C, Mg2+ as cation Yersinia pseudotuberculosis
0.43
-
 diphosphate pH 7.5, 30°C Yersinia pseudotuberculosis
0.43
-
 diphosphate diphosphate in form of magnesium diphosphate Yersinia pseudotuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requirement, CDPglucose synthesis and diphosphorolysis Yersinia pseudotuberculosis
Co2+ with decreasing order of efficiency in CDPglucose synthesis: Co2+, Mn2+, Mg2+ can replace Mg2+ in diphosphorolysis Yersinia pseudotuberculosis
Mg2+ requirement Yersinia pseudotuberculosis
Mg2+ with decreasing order of efficiency in CDPglucose synthesis: Co2+, Mn2+, Mg2+ Yersinia pseudotuberculosis
Mg2+ can replace Co2+ in diphosphorolysis Yersinia pseudotuberculosis
Mn2+ requirement Yersinia pseudotuberculosis
Mn2+ with decreasing order of efficiency in CDPglucose synthesis: Co2+, Mn2+, Mg2+, less effective in diphosphorolysis Yersinia pseudotuberculosis
additional information no activation by Ni2+ Yersinia pseudotuberculosis
additional information no activation by Cu2+ Yersinia pseudotuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 1 * 110000, SDS-PAGE Yersinia pseudotuberculosis
120000
-
 gel filtration Yersinia pseudotuberculosis

Organism

Organism UniProt Comment Textmining
Yersinia pseudotuberculosis
-
 type V, rough mutant, strain 25VO
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.143
-
-
 Yersinia pseudotuberculosis

Storage Stability

Storage Stability Organism
frozen, 2-3 months stable Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: ATP, GTP Yersinia pseudotuberculosis diphosphate + CDPglucose
-
 ?
CTP + alpha-D-glucose 1-phosphate no substrate in the forward reaction: TTP Yersinia pseudotuberculosis diphosphate + CDPglucose
-
 ?
diphosphate + CDP-glucose
-
 Yersinia pseudotuberculosis CTP + alpha-D-glucose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 110000, SDS-PAGE Yersinia pseudotuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Yersinia pseudotuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 10 broad, Mg2+ as activating cation Yersinia pseudotuberculosis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 rapid and irreversible inactivation below Yersinia pseudotuberculosis