Literature summary for 2.7.7.31 extracted from

Deshpande, S.; Yang, Y.; Chilkoti, A.; Zauscher, S.
Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase (2019), Methods Enzymol., 627, 163-188 .

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	used in assay conditions	Mus musculus
additional information	the enzyme can utilize several different metal ions including Mg2+, Co2+, Mn2+ and Zn2+, though its preference for nucleotide incorporation depends on the metal ion used. For example, when the buffer is supplemented with Mg2+, the purine nucleotide incorporation rate is 10fold higher than that of pyrimidines. When the buffer is supplemented with Co2+, the pyrimidine nucleotide incorporation rate is higher. Supplementing Mg2+ with sub-millimolar concentrations of Zn2+ increases the enzyme activity 2-4fold	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a 2'-deoxyribonucleoside 5'-triphosphate + single stranded DNAn	Mus musculus	-	diphosphate + single stranded DNAn+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	P09838	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a 2'-deoxyribonucleoside 5'-triphosphate + single stranded DNAn	-	Mus musculus	diphosphate + single stranded DNAn+1	-	?
B-dUTP + (Cy5-dT10)poly(dTTP)	-	Mus musculus	diphosphate + (Cy5-dT10)poly(dTTP)(B-dUTP)	-	?
dTTP + Cy5-dT10	-	Mus musculus	diphosphate + (Cy5-dT10)poly(dTTP)	-	?

Subunits

Subunits	Comment	Organism
?	x * 58000, SDS-PAGE	Mus musculus

Synonyms

Synonyms	Comment	Organism
TdT	-	Mus musculus
terminal deoxynucleotidyl transferase	-	Mus musculus

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Release 2023.1 (January 2023)