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Literature summary for 2.7.7.31 extracted from

  • Barthel, S.; Palluk, S.; Hillson, N.J.; Keasling, J.D.; Arlow, D.H.
    Enhancing terminal deoxynucleotidyl transferase activity on substrates with 3 terminal structures for enzymatic de novo DNA Synthesis (2020), Genes (Basel), 11, 102 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.25 mM used in assay conditions Mus musculus
Mg2+ 10 mM used in assay conditions Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn Mus musculus
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus P09838
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose resin column chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxyadenosine 5'-triphosphate + 5'-FAM labelled oligonucleotide
-
 Mus musculus diphosphate + ?
-
 ?
2',3'-dideoxycytosine 5'-triphosphate + 5'-FAM labelled oligonucleotide
-
 Mus musculus diphosphate + ?
-
 ?
2',3'-dideoxyguanosine 5'-triphosphate + 5'-FAM labelled oligonucleotide
-
 Mus musculus diphosphate + ?
-
 ?
2',3'-dideoxythymidine 5'-triphosphate + 5'-FAM labelled oligonucleotide
-
 Mus musculus diphosphate + ?
-
 ?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
-
 Mus musculus diphosphate + DNAn+1
-
 ?
additional information the wild type enzyme substantially reduces activity on primers with 3' terminal structure (hairpins) Mus musculus ?
-
-

Synonyms

Synonyms Comment Organism
TdT
-
 Mus musculus
terminal deoxynucleotidyl transferase
-
 Mus musculus