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Literature summary for 2.7.7.31 extracted from

  • Brodniewicz-Proba, T.; Buchowicz, J.
    Properties of a terminal deoxyribonucleotidyltransferase isolated from wheat germ (1980), Biochem. J., 191, 139-145.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.31

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requires both Mg2+ and Mn2+ Triticum aestivum
Mn2+ requires both Mn2+ and Mg2+ Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
500000
-
-
 Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn primer required Triticum aestivum diphosphate + DNAn+1 extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate ?
deoxynucleoside triphosphate + DNAn primer: polydeoxynucleotides Triticum aestivum diphosphate + DNAn+1 extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate ?
deoxynucleoside triphosphate + DNAn primer: oligodeoxynucleotides Triticum aestivum diphosphate + DNAn+1 extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate ?
deoxynucleoside triphosphate + DNAn polymerization is highly efficient if only one of the four deoxyribonucleoside 5'-triphosphates is present Triticum aestivum diphosphate + DNAn+1 extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate ?
deoxynucleoside triphosphate + DNAn prefers non-denatured to single-stranded DNA as primer Triticum aestivum diphosphate + DNAn+1 extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate ?
deoxynucleoside triphosphate + DNAn reaction is not template directed Triticum aestivum diphosphate + DNAn+1 extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate ?