Literature summary for 2.7.7.30 extracted from

Chin, Y.; Seo, N.; Kim, J.; Seo, J.
Metabolic engineering of Escherichia coli to produce 2'-fucosyllactose via salvage pathway of guanosine 5'-diphosphate (GDP)-l-fucose (2016), Biotechnol. Bioeng., 113, 2443-2452.

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Application

Application	Comment	Organism
synthesis	engineering of Escherichia coli for efficient production of 2'-fucosyllactose by introduction of the fkp gene coding for fucokinase/GDP-L-fucose diphosphorylase from Bacteroides fragilis and the fucT2 gene encoding alpha-1,2-fucosyltransferase from Helicobacter pylori to produce 2'-fucosyllactose from fucose, lactose and glycerol. The yield and productivity are further improved by deletion of the fucI-fucK gene cluster coding for fucose isomerase and fuculose kinase. Fed-batch fermentation of the final strain results in 23.1 g/l of extracellular 2'-fucosyllactose and a productivity of 0.39 g/l/h	Bacteroides fragilis

Cloned(Commentary)

Cloned (Comment)	Organism
-	Bacteroides fragilis

Organism

Organism	UniProt	Comment	Textmining
Bacteroides fragilis	Q58T34	-	-
Bacteroides fragilis ATCC 25285	Q58T34	-	-

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Release 2023.1 (January 2023)