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Literature summary for 2.7.7.30 extracted from

  • Kotake, T.; Hojo, S.; Tajima, N.; Matsuoka, K.; Koyama, T.; Tsumuraya, Y.
    A bifunctional enzyme with L-fucokinase and GDP-L-fucose pyrophosphorylase activities salvages free L-fucose in Arabidopsis (2008), J. Biol. Chem., 283, 8125-8135.
    View publication on PubMed
Search for more literature for 2.7.7.30

Cloned(Commentary)

Cloned (Comment) Organism
expression in BL21 (DE3) gold strain of Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
diphosphate addition of diphosphate strongly inhibits the formation of GDP-L-Fuc by rAtFKGPi Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 L-fucose-1-phosphate
-
 Arabidopsis thaliana
0.17
-
 GTP
-
 Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Arabidopsis thaliana
Mn2+ has only a poor effect on the GDP-L-Fuc pyrophosphorylase activity of rAtFKGP compared with Mg2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116400
-
 AtFKGP, calculated Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + L-fucose 1-phosphate Arabidopsis thaliana the monosaccharide L-fucose is found as a constituent of cell wall polysaccharides and sugar moieties of glycoproteins and has important physiological functions in seed plants diphosphate + GDP-L-fucose
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype Columbia (Col)
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose FF column, the recombinant protein fused to thioredoxin and His tags is partially purified by chelating chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cauline leaf mature plant Arabidopsis thaliana
-
cotyledon seedling Arabidopsis thaliana
-
flower mature plant Arabidopsis thaliana
-
flower bud mature plant, relatively high expression of AtFKGP mRNA Arabidopsis thaliana
-
inflorescence mature plant Arabidopsis thaliana
-
root seedling Arabidopsis thaliana
-
rosette leaf mature plant Arabidopsis thaliana
-
true leaf seedling Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.71
-
-
 Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-20°C, 50% (w/v) glycerol, 1 mM PMSF, 0.2 M NaCl, 50 mM Tris-HCl buffer, pH 7.5, 1 month, no loss of activity Arabidopsis thaliana
4°C, 1mM PMSF, 0.2 M NaCl, 50 mM Tris-HCl buffer, pH 7.5, 1 day, loss of more than 50% activity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + L-fucose 1-phosphate the monosaccharide L-fucose is found as a constituent of cell wall polysaccharides and sugar moieties of glycoproteins and has important physiological functions in seed plants Arabidopsis thaliana diphosphate + GDP-L-fucose
-
 ?
GTP + L-fucose 1-phosphate strict substrate specificity, no activity with L-Ara-1-P, Gal-1-P, Glc-1-P, Man-1-P, Xyl-1-P, GlcA-1-P, and Glc-NAc-1-P as glycosyl donor, and it uses specifically GTP as the nucleotide donor Arabidopsis thaliana diphosphate + GDP-L-fucose
-
 ?
L-fucose-1-phosphate + GTP
-
 Arabidopsis thaliana GDP-L-fucose + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
AtFKGP bifunctional enzyme with L-fucokinase and GDP-L-Fuc pyrophosphorylase Arabidopsis thaliana
GDP-L-Fuc pyrophosphorylase
-
 Arabidopsis thaliana
GDP-L-fucose pyrophosphorylase
-
 Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 rAtFKGP, GDP-L-Fuc pyrophosphorylase activity Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated, theoretical isoelectric point
-
 5.87