Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.30 extracted from

  • Ishihara, H.; Heath, E.C.
    The metabolism of L-fucose. IV. The biosynthesis of guanosine diphosphate L-fucose in porcine liver (1968), J. Biol. Chem., 243, 1110-1115.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
GDP-L-fucose
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Sus scrofa
Mg2+ divalent cation required, Mg2+ most effective Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, ammonium sulfate, calcium phosphate gel, Sephadex G-100, DEAE-cellulose, alumina C-gamma Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0036
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-16°C, 10 mM dithioerythritol, 24 hours, 30-35% loss of activity Sus scrofa
-16°C, 24 hours, 80% loss of activity Sus scrofa
0°C, 24 hours, 85% loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + GDP-L-fucose
-
Sus scrofa GTP + L-fucose 1-phosphate
-
r
GTP + alpha-D-mannose 1-phosphate 17.5% of activity with GDP-L-fucose Sus scrofa diphosphate + GDP-alpha-D-mannopyranose
-
?
GTP + L-fucose 1-phosphate specific for GTP Sus scrofa diphosphate + GDP-L-fucose
-
r
UTP + L-fucose 1-phosphate 18.7% of activity with GTP Sus scrofa diphosphate + UDP-L-fucose
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 approx. 85% of maximal activity at pH 6.0, approx. 70% of maximal activity at pH 9.5 Sus scrofa