Literature summary for 2.7.7.3 extracted from

Wubben, T.J.; Mesecar, A.D.
Kinetic, thermodynamic, and structural insight into the mechanism of phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis (2010), J. Mol. Biol., 404, 202-219.

Search for more literature for 2.7.7.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with pantetheine 4'-phosphate and adenosine-5'-[(alpha,beta)-methyleno]triphosphate, sitting drop vapor diffusion method, using 38% (v/v) polyethylene glycol 200 and 0.1 M HEPES (pH 7.2), at 21°C	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
adenosine-5'-[(alpha,beta)-methyleno]triphosphate	-	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0067	-	3'-dephospho-CoA	apparent value, at 25°C in 50 mM Tris (pH 8.0)	Mycobacterium tuberculosis
0.0377	-	pantetheine 4'-phosphate	apparent value, at 25°C in 75 mM HEPES (pH 7.0)	Mycobacterium tuberculosis
0.1157	-	ATP	apparent value, at 25°C in 75 mM HEPES (pH 7.0)	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WPA5	-	-
Mycobacterium tuberculosis H37Rv	P9WPA5	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography and Superdex 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pantetheine 4'-phosphate	-	Mycobacterium tuberculosis	diphosphate + 3'-dephospho-CoA	-	r
ATP + pantetheine 4'-phosphate	-	Mycobacterium tuberculosis H37Rv	diphosphate + 3'-dephospho-CoA	-	r
diphosphate + 3'-dephospho-CoA	-	Mycobacterium tuberculosis	ATP + pantetheine 4'-phosphate	-	r
diphosphate + 3'-dephospho-CoA	-	Mycobacterium tuberculosis H37Rv	ATP + pantetheine 4'-phosphate	-	r

Subunits

Subunits	Comment	Organism
homohexamer	x-ray crystallography	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
phosphopantetheine adenylyltransferase	-	Mycobacterium tuberculosis
PPAT	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.547	-	ATP	at 25°C in 75 mM HEPES (pH 7.0)	Mycobacterium tuberculosis
4.808	-	3'-dephospho-CoA	at 25°C in 50 mM Tris (pH 8.0)	Mycobacterium tuberculosis
4.853	-	pantetheine 4'-phosphate	at 25°C in 75 mM HEPES (pH 7.0)	Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.388	-	adenosine-5'-[(alpha,beta)-methyleno]triphosphate	at 25°C in 75 mM HEPES (pH 7.0)	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)