Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with pantetheine 4'-phosphate and adenosine-5'-[(alpha,beta)-methyleno]triphosphate, sitting drop vapor diffusion method, using 38% (v/v) polyethylene glycol 200 and 0.1 M HEPES (pH 7.2), at 21°C | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine-5'-[(alpha,beta)-methyleno]triphosphate | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
3'-dephospho-CoA | apparent value, at 25°C in 50 mM Tris (pH 8.0) | Mycobacterium tuberculosis | |
0.0377 | - |
pantetheine 4'-phosphate | apparent value, at 25°C in 75 mM HEPES (pH 7.0) | Mycobacterium tuberculosis | |
0.1157 | - |
ATP | apparent value, at 25°C in 75 mM HEPES (pH 7.0) | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPA5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPA5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pantetheine 4'-phosphate | - |
Mycobacterium tuberculosis | diphosphate + 3'-dephospho-CoA | - |
r | |
ATP + pantetheine 4'-phosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + 3'-dephospho-CoA | - |
r | |
diphosphate + 3'-dephospho-CoA | - |
Mycobacterium tuberculosis | ATP + pantetheine 4'-phosphate | - |
r | |
diphosphate + 3'-dephospho-CoA | - |
Mycobacterium tuberculosis H37Rv | ATP + pantetheine 4'-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
phosphopantetheine adenylyltransferase | - |
Mycobacterium tuberculosis |
PPAT | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.547 | - |
ATP | at 25°C in 75 mM HEPES (pH 7.0) | Mycobacterium tuberculosis | |
4.808 | - |
3'-dephospho-CoA | at 25°C in 50 mM Tris (pH 8.0) | Mycobacterium tuberculosis | |
4.853 | - |
pantetheine 4'-phosphate | at 25°C in 75 mM HEPES (pH 7.0) | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.388 | - |
adenosine-5'-[(alpha,beta)-methyleno]triphosphate | at 25°C in 75 mM HEPES (pH 7.0) | Mycobacterium tuberculosis |