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Literature summary for 2.7.7.28 extracted from
- Purification and properties of guanosine diphosphate hexose pyrophosphorylase from mammalian tissues (1966), J. Biol. Chem., 241, 2007-2013.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-mannose 1-phosphate | strong | Bos taurus |  |
| GDPmannose | strong | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
GDPmannose | pH 7.8, 25°C | Bos taurus | |
| 0.082 | - |
diphosphate | pH 7.8, 25°C | Bos taurus | |
| 0.62 | - |
GDPglucose | pH 7.8, 25°C | Bos taurus | |
| 2.6 | - |
GTP | pH 7.8, 25°C | Bos taurus | |
| 2.6 | - |
D-glucose 1-phosphate | pH 7.8, 25°C | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
| cytoplasm | - |
Bos taurus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | metal requirement can be met by Co2+ | Bos taurus | |
| Mg2+ | requirement, maximal activity at about 5 mM, maximal activity at about twice the diphosphate-concentration | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-glucose + diphosphate | Bos taurus | best substrate | GTP + D-glucose 1-phosphate | - |
r | |
| GDPmannose + diphosphate | Bos taurus | - |
GTP + D-mannose 1-phosphate | - |
r | |
| GDPmannose + diphosphate | Rattus norvegicus | reaction at about half the rate of GDPglucose | GTP + D-mannose 1-phosphate | - |
? | |
| IDP-glucose + diphosphate | Bos taurus | reaction at 21% the rate of GDPglucose | ITP + D-glucose 1-phosphate | - |
r | |
| IDPmannose + diphosphate | Bos taurus | - |
ITP + D-mannose 1-phosphate | - |
r | |
| IDPmannose + diphosphate | Rattus norvegicus | reaction at 7% the rate of GDPglucose | ITP + D-mannose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| mammary gland | - |
Rattus norvegicus | - |
| muscle | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.38 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, at pH 6-6.5, t1/2: 60 days | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-glucose + diphosphate | best substrate | Bos taurus | GTP + D-glucose 1-phosphate | - |
r | |
| GDPmannose + diphosphate | - |
Bos taurus | GTP + D-mannose 1-phosphate | - |
r | |
| GDPmannose + diphosphate | reaction at about half the rate of GDPglucose | Rattus norvegicus | GTP + D-mannose 1-phosphate | - |
? | |
| IDP-glucose + diphosphate | reaction at 21% the rate of GDPglucose | Bos taurus | ITP + D-glucose 1-phosphate | - |
r | |
| IDPmannose + diphosphate | - |
Bos taurus | ITP + D-mannose 1-phosphate | - |
r | |
| IDPmannose + diphosphate | reaction at 7% the rate of GDPglucose | Rattus norvegicus | ITP + D-mannose 1-phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
15% loss of activity after 5 min, 33% loss of activity after 10 min | Bos taurus |
| 55 | - |
1 min, 57% loss of activity | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 40% of maximal activity, pH 9.0: about half-maximal activity | Rattus norvegicus |
| 6.5 | 9 | pH 6.5: about 40% of maximal activity, pH 9.0: about half-maximal activity | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | t1/2: 60 days at 4°C | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00017 | - |
GDP-mannose | pH 7.8, 25°C | Bos taurus | |
| 0.047 | - |
D-mannose 1-phosphate | pH 7.8, 25°C | Bos taurus | |
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