Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.27 extracted from

  • Tuncel, A.; Kawaguchi, J.; Ihara, Y.; Matsusaka, H.; Nishi, A.; Nakamura, T.; Kuhara, S.; Hirakawa, H.; Nakamura, Y.; Cakir, B.; Nagamine, A.; Okita, T.W.; Hwang, S.K.; Satoh, H.
    The rice endosperm ADP-glucose pyrophosphorylase large subunit is essential for optimal catalysis and allosteric regulation of the heterotetrameric enzyme (2014), Plant Cell Physiol., 55, 1169-1183.
    View publication on PubMed
Search for more literature for 2.7.7.27

Protein Variants

Protein Variants Comment Organism
A171V mutation in large subunit gene AGPL2. Seeds of mutant plants are severely shriveled and have seed weight and starch content comparable with the shriveled seeds from AGPL2 null mutants. The catalytic and allosteric regulatory properties of the mutant enzyme are significantly impaired, showing lower specific activities and affinities for the activator 3-phosphoglycerate Oryza sativa
T139I mutation in large subunit gene AGPL2. Seeds of mutant plants are severely shriveled and have seed weight and starch content comparable with the shriveled seeds from AGPL2 null mutants. The catalytic and allosteric regulatory properties of the mutant enzyme are significantly impaired, showing lower specific activities and affinities for the activator 3-phosphoglycerate Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
 alpha-D-glucose 1-phosphate S0.5 value, wild-type, 37°C, pH 7.0 Oryza sativa
0.49
-
 ATP S0.5 value, large subunit mutant T139I with wild-type small subunit, 37°C, pH 7.0 Oryza sativa
0.5
-
 ATP S0.5 value, wild-type small subunit alone, 37°C, pH 7.0 Oryza sativa
0.51
-
 ATP S0.5 value, large subunit mutant A171V with wild-type small subunit, 37°C, pH 7.0 Oryza sativa
0.58
-
 ATP S0.5 value, wild-type, 37°C, pH 7.0 Oryza sativa
0.72
-
 alpha-D-glucose 1-phosphate S0.5 value, large subunit mutant A171V with wild-type small subunit, 37°C, pH 7.0 Oryza sativa
1.32
-
 alpha-D-glucose 1-phosphate S0.5 value, wild-type small subunit alone, 37°C, pH 7.0 Oryza sativa
1.43
-
 alpha-D-glucose 1-phosphate S0.5 value, large subunit mutant T139I with wild-type small subunit, 37°C, pH 7.0 Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ S0.6 value for wild-type 2.76 mM, large subunit mutant T139I with wild-type small subunit, 3.81 mM, large subunit mutant A171V with wild-type small subunit, 4.34 mM Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Oryza sativa diphosphate + ADP-glucose
-
 ?

Synonyms

Synonyms Comment Organism
AGPL2 large subunit Oryza sativa
AGPS2b small subunit Oryza sativa