Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | activates GlgC by twofold, but has no effect on GlgC/GlgD complex. In the presence of D-fructose 1,6-bisphosphate GlgC becomes sensitive to inhibitors phosphoenolpyruvate and inorganic salts | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | inhibits both subunit GlgC and GlgC/GlgD complex | Streptococcus mutans | |
KCl | inhibits GlgC/GlgD complex, no effect on subunit GlgC alone | Streptococcus mutans | |
NaCl | inhibits GlgC/GlgD complex, no effect on subunit GlgC alone | Streptococcus mutans | |
phosphate | inhibits both subunit GlgC and GlgC/GlgD complex | Streptococcus mutans | |
phosphoenolpyruvate | inhibitory to GlgC/GlgD complex, inhibitory to subunit GlgC only in presence of fructose 1,6-bisphosphate | Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
alpha-D-glucose 1-phosphate | S0.5 value, Hill coefficient 1.2, GlgC/GlgD complex, pH 8.0, 37°C | Streptococcus mutans | |
0.37 | - |
ATP | S0.5 value, Hill coefficient 1.5, subunit GlgC in presence of fructose 1,6-bisphosphate, pH 8.0, 37°C | Streptococcus mutans | |
1.18 | - |
ATP | S0.5 value, Hill coefficient 3.7, GlgC/GlgD complex, pH 8.0, 37°C | Streptococcus mutans | |
2.04 | - |
alpha-D-glucose 1-phosphate | S0.5 value, Hill coefficient 1.7, subunit GlgC in presence of fructose 1,6-bisphosphate, pH 8.0, 37°C | Streptococcus mutans | |
3.42 | - |
ATP | S0.5 value, Hill coefficient 1.2, subunit GlgC, pH 8.0, 37°C | Streptococcus mutans | |
3.44 | - |
alpha-D-glucose 1-phosphate | S0.5 value, Hill coefficient 0.9, subunit GlgC, pH 8.0, 37°C | Streptococcus mutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | subunit GlgC, S0.5 value 1.55 mM and Hill coefficient 1.7, complex of GlgC/GlgD, S0.5 value 2.36, hill coefficient 1.6 | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | M9TIK1 and M9TG57 | M9TIK1 i.e. subunit GlgC, M9TG57 i.e. subunit GlgD | - |
Streptococcus mutans ATCC 25175 | M9TIK1 and M9TG57 | M9TIK1 i.e. subunit GlgC, M9TG57 i.e. subunit GlgD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Streptococcus mutans | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Streptococcus mutans ATCC 25175 | diphosphate + ADP-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlgC | - |
Streptococcus mutans |
GlgD | - |
Streptococcus mutans |
General Information | Comment | Organism |
---|---|---|
physiological function | when expressed individually, subunit GlgC has ADP-Glc diphosphorylase activity, whereas subunit GlgD is inactive. The coexpressed GlgC/GlgD protein is one order of magnitude more active than subunit GlgC alone. Fructose-1,6-bis-phosphate activates GlgC by twofold, but has no effect on GlgC/GlgD. Phosphoenolpyruvate and inorganic salts inhibit GlgC/GlgD without affecting GlgC. In the presence of fructose-1,6-bis-phosphate GlgC acquires a GlgC/GlgD-like behaviour, becoming sensitive to the stated inhibitors | Streptococcus mutans |