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Literature summary for 2.7.7.27 extracted from

  • Boehlein, S.K.; Shaw, J.R.; Hwang, S.K.; Stewart, J.D.; Curtis Hannah, L.
    Deciphering the kinetic mechanisms controlling selected plant ADP-glucose pyrophosphorylases (2013), Arch. Biochem. Biophys., 535, 215-226.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate in presence of 3-phosphoglycerate, enzyme follows a Theorell-Chance bi-bi mechanism with ATP binding first and ADP-Glc releasing last. 3-Phosphoglycerate increases the binding affinity for both substrates with little effect on velocity for the maize and chimeric maize/potato isoforms. Activation constant Ka is 0.06 for maize enzyme and 0.03 for the chimeric enzyme Zea mays
3-phosphoglycerate presence of 3-phosphoglycerate increases the velocity and the affinity for glucose 1-phosphate for the potato enzymes. Redox state does not affect kcat of the two potato isoforms. Without 3-phosphoglycerate the oxidized potato enzyme exhibits a rapid equilibrium random bi-bi mechanism with a dead end ternary complex. Activation constant Ka is 0.52 for the oxidized and 0.20 for the reduced enzyme and 0.03 for the chimeric enzyme Solanum tuberosum
fructose 6-phosphate activation constant Ka is 0.53 for maize enzyme and 0.15 for the chimeric enzyme Zea mays
fructose 6-phosphate activation constant Ka is 9.8 for the oxidized and 20 for the reduced enzyme and 0.15 for the chimeric enzyme, respectively Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
additional information construction of a hybrid AGPase composed of portions from the maize endosperm and the potato tuber AGPases in the small subunit paired with a wild-type maize large subunit. The chimeric maize/potato heat stable enzyme lacks the cysteine responsible for redox changes Solanum tuberosum
additional information construction of a hybrid AGPase composed of portions from the maize endosperm and the potato tuber AGPases in the small subunit paired with a wild-type maize large subunit. The chimeric maize/potato heat stable enzyme lacks the cysteine responsible for redox changes Zea mays

Inhibitors

Inhibitors Comment Organism Structure
ADP-alpha-D-glucose in wild-type, competitive with ATP, noncompetitive with glucose 1-phosphate. In chimeric mutant, competitive with ATP, mixed type inhibition with glucose 1-phosphate Solanum tuberosum
ADP-alpha-D-glucose in wild-type, competitive with ATP, noncompetitive with glucose 1-phosphate. In chimeric mutant, competitive with ATP, mixed type inhibition with glucose 1-phosphate Zea mays
diphosphate in wild-type, mixed type inhibition with ATP, competitive with glucose 1-phosphate. In chimeric mutant, mixed type inhibition with ATP, competitive with glucose 1-phosphate Solanum tuberosum
diphosphate in wild-type, mixed type inhibition with ATP, competitive with glucose 1-phosphate. In chimeric mutant, mixed type inhibition with ATP, competitive with glucose 1-phosphate Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 alpha-D-glucose 1-phosphate chimeric enzyme, pH 7.4, 37°C, presence of 3-phosphoglycerate Solanum tuberosum
0.017
-
 alpha-D-glucose 1-phosphate chimeric enzyme, pH 7.4, 37°C, presence of 3-phosphoglycerate Zea mays
0.04
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.4, 37°C, presence of 3-phosphoglycerate Zea mays
0.05
-
 alpha-D-glucose 1-phosphate reduced wild-type, pH 7.4, 37°C, presence of 3-phosphoglycerate Solanum tuberosum
0.053
-
 ATP chimeric enzyme, pH 7.4, 37°C, presence of 3-0.040 phosphoglycerate Solanum tuberosum
0.053
-
 ATP chimeric enzyme, pH 7.4, 37°C, presence of 3-phosphoglycerate Zea mays
0.1
-
 alpha-D-glucose 1-phosphate oxidized wild-type, pH 7.4, 37°C, presence of 3-phosphoglycerate Solanum tuberosum
0.11
-
 ATP wild-type, pH 7.4, 37°C, presence of 3-phosphoglycerate Zea mays
0.13
-
 ATP oxidized wild-type, pH 7.4, 37°C, presence of 3-phosphoglycerate Solanum tuberosum
0.17
-
 ATP oxidized wild-type, pH 7.4, 37°C Solanum tuberosum
0.17
-
 ATP oxidized wild-type, pH 7.4, 37°C, presence of phosphate Solanum tuberosum
0.19
-
 ATP reduced wild-type, pH 7.4, 37°C, presence of 3-phosphoglycerate Solanum tuberosum
0.29
-
 ATP reduced wild-type, pH 7.4, 37°C, presence of phosphate Solanum tuberosum
0.3
-
 ATP chimeric enzyme, pH 7.4, 37°C, presence of phosphate Solanum tuberosum
0.3
-
 ATP chimeric enzyme, pH 7.4, 37°C, presence of phosphate Zea mays
0.31
-
 ATP reduced wild-type, pH 7.4, 37°C Solanum tuberosum
0.58
-
 ATP wild-type, pH 7.4, 37°C, presence of phosphate Zea mays
0.9
-
 ATP chimeric enzyme, pH 7.4, 37°C Solanum tuberosum
0.9
-
 ATP chimeric enzyme, pH 7.4, 37°C Zea mays
1.07
-
 alpha-D-glucose 1-phosphate reduced wild-type, pH 7.4, 37°C Solanum tuberosum
1.63
-
 alpha-D-glucose 1-phosphate oxidzed wild-type, pH 7.4, 37°C Solanum tuberosum
1.81
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.4, 37°C, presence of phosphate Zea mays
2.8
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.4, 37°C Zea mays
4
-
 ATP wild-type, pH 7.4, 37°C Zea mays
4.01
-
 alpha-D-glucose 1-phosphate oxidized wild-type, pH 7.4, 37°C, presence of phosphate Solanum tuberosum
5.18
-
 alpha-D-glucose 1-phosphate reduced wild-type, pH 7.4, 37°C, presence of phosphate Solanum tuberosum
6.79
-
 alpha-D-glucose 1-phosphate chimeric enzyme, pH 7.4, 37°C, presence of phosphate Solanum tuberosum
6.79
-
 alpha-D-glucose 1-phosphate chimeric enzyme, pH 7.4, 37°C, presence of phosphate Zea mays
11.9
-
 alpha-D-glucose 1-phosphate chimeric enzyme, pH 7.4, 37°C Solanum tuberosum
11.9
-
 alpha-D-glucose 1-phosphate chimeric enzyme, pH 7.4, 37°C Zea mays

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-
Zea mays Q947C0 and P55241 Q947C0, i.e. small subunit Bt2, P55241 i.e. large subunit Sh2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?
ATP + alpha-D-glucose 1-phosphate
-
 Zea mays diphosphate + ADP-glucose
-
 ?
additional information in presence of 3-phosphoglycerate, enzyme follows a Theorell-Chance bi-bi mechanism with ATP binding first and ADP-Glc releasing last. 3-Phosphoglycerate increases the binding affinity for both substrates with little effect on velocity for the maize and chimeric maize/potato isoforms Zea mays ?
-
 ?
additional information presence of 3-phosphoglycerate increases the velocity and the affinity for glucose 1-phosphate for the potato enzymes. Redox state does not affect kcat of the two potato isoforms. Without 3-phosphoglycerate the oxidized potato enzyme exhibits a rapid equilibrium random bi-bi mechanism with a dead end ternary complex Solanum tuberosum ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 3.7 ATP wild-type, pH 7.4, 37°C, presence of phosphate Zea mays
3.9
-
 ATP oxidized wild-type, pH 7.4, 37°C, presence of phosphate Solanum tuberosum
5.4
-
 ATP reduced wild-type, pH 7.4, 37°C, presence of phosphate Solanum tuberosum
13.7
-
 ATP reduced wild-type, pH 7.4, 37°C Solanum tuberosum
15.4
-
 ATP oxidized wild-type, pH 7.4, 37°C Solanum tuberosum
35.2
-
 ATP wild-type, pH 7.4, 37°C Zea mays
42.6
-
 ATP wild-type, pH 7.4, 37°C, presence of 3-phosphoglycerate Zea mays
47.5
-
 ATP chimeric enzyme, pH 7.4, 37°C, presence of 3-phosphoglycerate Solanum tuberosum
47.5
-
 ATP chimeric enzyme, pH 7.4, 37°C, presence of 3-phosphoglycerate Zea mays
67.6
-
 ATP chimeric enzyme, pH 7.4, 37°C Solanum tuberosum
67.6
-
 ATP chimeric enzyme, pH 7.4, 37°C Zea mays
70.6
-
 ATP chimeric enzyme, pH 7.4, 37°C, presence of phosphate Solanum tuberosum
70.6
-
 ATP chimeric enzyme, pH 7.4, 37°C, presence of phosphate Zea mays
147.7
-
 ATP reduced wild-type, pH 7.4, 37°C, presence of 3-phosphoglycerate Solanum tuberosum
163.1
-
 ATP oxidized wild-type, pH 7.4, 37°C, presence of 3-phosphoglycerate Solanum tuberosum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
 ADP-alpha-D-glucose wild-type, substrate ATP, pH 7.4, 37°C Zea mays
0.01
-
 ADP-alpha-D-glucose chimeric mutant, substrate ATP, pH 7.4, 37°C Zea mays
0.025
-
 diphosphate reduced wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.03
-
 diphosphate chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Zea mays
0.045
-
 ADP-alpha-D-glucose oxidized wild-type, substrate ATP, pH 7.4, 37°C Solanum tuberosum
0.047
-
 diphosphate oxidized wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.047
-
 diphosphate oxidized wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.047
-
 diphosphate reduced wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.05
-
 ADP-alpha-D-glucose chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.05
-
 ADP-alpha-D-glucose chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Zea mays
0.075
-
 diphosphate chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Zea mays
0.083
-
 ADP-alpha-D-glucose chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.083
-
 ADP-alpha-D-glucose chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Zea mays
0.09
-
 ADP-alpha-D-glucose chimeric mutant, substrate glucose 1-phosphate, pH 7.4, 37°C Solanum tuberosum
0.09
-
 ADP-alpha-D-glucose chimeric mutant, substrate glucose 1-phosphate, pH 7.4, 37°C Zea mays
0.09
-
 ADP-alpha-D-glucose wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C Zea mays
0.1
-
 diphosphate wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Zea mays
0.11
-
 ADP-alpha-D-glucose oxidized wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C Solanum tuberosum
0.11
-
 ADP-alpha-D-glucose oxidized wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.11
-
 diphosphate wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Zea mays
0.12
-
 ADP-alpha-D-glucose wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Zea mays
0.13
-
 ADP-alpha-D-glucose oxidized wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.14
-
 ADP-alpha-D-glucose reduced wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.19
-
 ADP-alpha-D-glucose chimeric mutant, substrate ATP, pH 7.4, 37°C Solanum tuberosum
0.19
-
 diphosphate oxidized wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C Solanum tuberosum
0.19
-
 diphosphate wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C Zea mays
0.21
-
 ADP-alpha-D-glucose reduced wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.35
-
 diphosphate chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.35
-
 diphosphate reduced wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C Solanum tuberosum
0.36
-
 diphosphate chimeric mutant, substrate ATP, pH 7.4, 37°C Solanum tuberosum
0.36
-
 diphosphate chimeric mutant, substrate ATP, pH 7.4, 37°C Zea mays
0.44
-
 diphosphate chimeric mutant, substrate glucose 1-phosphate, pH 7.4, 37°C Solanum tuberosum
0.44
-
 diphosphate chimeric mutant, substrate glucose 1-phosphate, pH 7.4, 37°C Zea mays
0.46
-
 ADP-alpha-D-glucose reduced wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C Solanum tuberosum
0.55
-
 diphosphate chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C Solanum tuberosum
0.55
-
 diphosphate reduced wild-type, substrate ATP, pH 7.4, 37°C Solanum tuberosum
0.81
-
 diphosphate oxidized wild-type, substrate ATP, pH 7.4, 37°C Solanum tuberosum
0.9
-
 diphosphate wild-type, substrate ATP, pH 7.4, 37°C Zea mays
1.2
-
 ADP-alpha-D-glucose wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C Zea mays