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Literature summary for 2.7.7.27 extracted from

  • Machtey, M.; Kuhn, M.L.; Flasch, D.A.; Aleanzi, M.; Ballicora, M.A.; Iglesias, A.A.
    Insights into glycogen metabolism in chemolithoautotrophic bacteria from distinctive kinetic and regulatory properties of ADP-glucose pyrophosphorylase from Nitrosomonas europaea (2012), J. Bacteriol., 194, 6056-6065.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
oxaloacetate
-
 Nitrosomonas europaea
phosphoenolpyruvate
-
 Nitrosomonas europaea
pyruvate
-
 Nitrosomonas europaea

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nitrosomonas europaea

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
 Nitrosomonas europaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, presence of Mg2+ Nitrosomonas europaea
0.13
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, presence of Co2+ Nitrosomonas europaea
0.13
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, presence of Mn2+ Nitrosomonas europaea
0.72
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C, presence of Cd2+ Nitrosomonas europaea

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ S0.5 value 0.74 mM in direction of synthesis of ADP-glucose Nitrosomonas europaea
Co2+ S0.5 value 0.46 mM in direction of synthesis of ADP-glucose Nitrosomonas europaea
Mg2+ S0.5 value above 40 mM in direction of synthesis of ADP-glucose Nitrosomonas europaea
Mn2+ S0.5 value 1.37 mM in direction of synthesis of ADP-glucose Nitrosomonas europaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 4 * 49200, calculated, 4 * 49000, SDS-PAGE Nitrosomonas europaea
49200
-
 4 * 49200, calculated, 4 * 49000, SDS-PAGE Nitrosomonas europaea
200000
-
 gel filtration Nitrosomonas europaea

Organism

Organism UniProt Comment Textmining
Nitrosomonas europaea Q82T88
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Nitrosomonas europaea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
 pH 8.0, 37°C Nitrosomonas europaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate the catalytic efficiency for ATP is not dependent upon the divalent metal ion Nitrosomonas europaea diphosphate + ADP-glucose
-
 r
ATP + alpha-D-mannose 1-phosphate the catalytic efficiency with glucose 1-phosphate is at least one order of magnitude higher than that with mannose 1-phosphate Nitrosomonas europaea diphosphate + ADP-mannose
-
 r
additional information enzyme is able to accept UTP, CTP, dTTP and GTP with catalytic efficiencies below 1% of that with ATP Nitrosomonas europaea ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 49200, calculated, 4 * 49000, SDS-PAGE Nitrosomonas europaea

Synonyms

Synonyms Comment Organism
GlgC
-
 Nitrosomonas europaea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 50 fully active between 0°C and 50°C for 15 min Nitrosomonas europaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Nitrosomonas europaea

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.8 9.1 stable for 30 min at 37°C Nitrosomonas europaea

pI Value

Organism Comment pI Value Maximum pI Value
Nitrosomonas europaea calculated
-
 5.8