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Literature summary for 2.7.7.27 extracted from

  • Hwang, S.K.; Nagai, Y.; Kim, D.; Okita, T.W.
    Direct appraisal of the potato tuber ADP-glucose pyrophosphorylase large subunit in enzyme function by study of a novel mutant form (2008), J. Biol. Chem., 283, 6640-6647.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate allosterin activator Solanum tuberosum

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant subunits and tetramers, overview Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
additional information construction of a LRKNSWT heterotetramer, the LS mutant homotetramer LRKN is catalytically very inefficient, binds ATP less efficiently, and is less heat-stable, overview Solanum tuberosum
S302N site-directed mutagenesis, the mutant shows increased solubility of the recombinant potato tuber large subunit and, in turn, enabling it to form a homotetrameric structure. The LS302N homotetramer possesses very little enzyme activity at a level 100fold less than that seen for the unactivated small subunit homotetramer. Unlike the small subunit enzyme, the LS302N homotetramer enzyme is neither activated by the effector 3-phosphoglycerate nor inhibited by phosphate. The mutation significantly enhances glycogen production in bacterial host cells Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
phosphate allosteric inhibitor Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of wild-type and mutant large subunits, overview Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate Solanum tuberosum
-
 ADP-D-glucose + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-N3-ATP + alpha-D-glucose 1-phosphate both the large and small subunit in the heterotetrameric form are labeled at equivalent rates with 8-N3-ATP, an analog which can readily substitute for ATP in catalysis Solanum tuberosum 8-N3-ADP-D-glucose + diphosphate
-
 r
ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum ADP-D-glucose + diphosphate
-
 r
ATP + alpha-D-glucose 1-phosphate the small subunit displays both catalytic and regulatory properties, the large subunit possesses catalytic and regulatory properties only when assembled with small subunit, the net properties of the heterotetrameric enzyme is a product of subunit synergy, overview Solanum tuberosum ADP-D-glucose + diphosphate
-
 r

Subunits

Subunits Comment Organism
More higher plant ADP-glucose pyrophosphorylase is a heterotetramer consisting of two subunit types, which have evolved at different rates from a common ancestral gene. The potato tuber small subunit displays both catalytic and regulatory properties. The large subunit possesses catalytic and regulatory properties only when assembled with small subunit, the net properties of the heterotetrameric enzyme is a product of subunit synergy, modeling of AGPase structures, overview Solanum tuberosum

Synonyms

Synonyms Comment Organism
ADP-glucose pyrophosphorylase
-
 Solanum tuberosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, the recombinant LRKNSWT heterotetrameric mutant retains 92% of wild-type AGPase activity, the recombinant SWT homotetrameric mutant has an intermediate level of 68%, whereas the recombinant homotetrameric mutant LRKN shows only below 7% of wild-type activity Solanum tuberosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
121
-
 diphosphate 37°C, wild-type large subunit Solanum tuberosum
139
-
 ADP-glucose 37°C, wild-type large subunit Solanum tuberosum
158
-
 alpha-D-glucose 1-phosphate 37°C, wild-type large subunit Solanum tuberosum
166
-
 ATP 37°C, wild-type large subunit Solanum tuberosum