Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | wild-type, half-maximal activation at 0.016 mM, mutant A33K, at 0.0055 mM, mutant G96N, at 0.0064 mM, mutant A33K/G96N, at 0.0066 mM | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
A33K | mutation in leaf-specific large subunit ApL1, little effect on affinity of holoenzyme for substrates | Arabidopsis thaliana |
A33K/G96N | mutation in leaf-specific large subunit ApL1, little effect on affinity of holoenzyme for substrates. Double mutant is less susceptible for inhibition by phosphate and shows increased sensitivity to 3-phosphoglycerate | Arabidopsis thaliana |
G96N | mutation in leaf-specific large subunit ApL1, little effect on affinity of holoenzyme for substrates | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | wild-type, 50% inhibition at 0.044 mM, mutant A33K, 50% inhibition at 0.043 mM, mutant G96N, 50% inhibition at 0.054 mM, mutant A33K/G96N, 50% inhibition at 0.268 mM, all in presence of 3-phosphoglycerate in at concentration of halfmaximal activation | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
alpha-D-glucose-1-phosphate | mutant A33K, pH 7.5 | Arabidopsis thaliana | |
0.052 | - |
ATP | mutant G96N, pH 7.5 | Arabidopsis thaliana | |
0.055 | - |
ATP | mutant A33K/G96N, pH 7.5 | Arabidopsis thaliana | |
0.055 | - |
alpha-D-glucose-1-phosphate | mutant G96N, pH 7.5 | Arabidopsis thaliana | |
0.056 | - |
ATP | mutant A33K, pH 7.5 | Arabidopsis thaliana | |
0.063 | - |
alpha-D-glucose-1-phosphate | mutant A33K/G96N, pH 7.5 | Arabidopsis thaliana | |
0.073 | - |
ATP | wild-type, pH 7.5 | Arabidopsis thaliana | |
0.083 | - |
alpha-D-glucose-1-phosphate | wild-type, pH 7.5 | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose-1-phosphate | - |
Arabidopsis thaliana | diphosphate + ADP-glucose | - |
? |