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Literature summary for 2.7.7.27 extracted from

  • Hwang, S.K.; Hamada, S.; Okita, T.W.
    Catalytic implications of the higher plant ADP-glucose pyrophosphorylase large subunit (2007), Phytochemistry, 68, 464-477.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate 50% of maximal activation for wild-type at 0.11 mM, for mutant P17L at 0.08 mM, for mutant P26L at 0.15 mM, for mutant P44L at 0.25 mM, for mutant P52L at 0.77 mM, for mutant P55L at 0.26 mM, for mutant P66L at 0.03 mM Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
LP17L site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
LP26L site-directed mutagenesis, the mutant shows slightly reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
LP44E site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
LP44K site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
LP44L site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
LP44R site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
LP44S site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
LP44Y site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
LP55L site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
LP66L site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme Escherichia coli
P17L mutation in large enzyme subunit, moderate effect on catalytic properties Solanum tuberosum
P26L mutation in large enzyme subunit, moderate effect on catalytic properties but severely impaired catalytic rates Solanum tuberosum
P44L mutation in large enzyme subunit, moderate changes in properties toward 3-phosphoglycerate Solanum tuberosum
P52L mutation in large enzyme subunit, down-regulatory properties toward 3-phosphoglycerate Solanum tuberosum
P55L mutation in large enzyme subunit, moderate effect on catalytic properties Solanum tuberosum
P66L mutation in large enzyme subunit, up-regulatory properties toward 3-phosphoglycerate Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics wild-type and mutant enzymes, photoaffinity labeling of the AGPase heterotetramers, overview Escherichia coli
0.09
-
 alpha-D-glucose 1-phosphate mutant P26L, 37°C Solanum tuberosum
0.12
-
 alpha-D-glucose 1-phosphate wild-type, 37°C Solanum tuberosum
0.14
-
 alpha-D-glucose 1-phosphate mutant P55L, 37°C Solanum tuberosum
0.14
-
 alpha-D-glucose 1-phosphate mutant P66L, 37°C Solanum tuberosum
0.15
-
 alpha-D-glucose 1-phosphate mutant P52L, 37°C Solanum tuberosum
0.16
-
 alpha-D-glucose 1-phosphate mutant P17L, 37°C Solanum tuberosum
0.19
-
 ATP wild-type, 37°C Solanum tuberosum
0.2
-
 ATP mutant P66L, 37°C Solanum tuberosum
0.21
-
 ATP mutant P17L, 37°C Solanum tuberosum
0.27
-
 ATP mutant P52L, 37°C Solanum tuberosum
0.33
-
 ATP mutant P55L, 37°C Solanum tuberosum
0.68
-
 ATP mutant P44L, 37°C Solanum tuberosum
0.83
-
 alpha-D-glucose 1-phosphate mutant P44L, 37°C Solanum tuberosum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km value 2.4 mM for wild-type Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli ER2566
-
-
-
Solanum tuberosum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 catalytic efficiency of wild-type and mutant enzymes, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
ADP-glucose pyrophosphorylase
-
 Escherichia coli
AGPase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
 ATP mutant P44L, 37°C Solanum tuberosum
33
-
 ATP mutant P52L, 37°C Solanum tuberosum
49
-
 ATP mutant P55L, 37°C Solanum tuberosum
62
-
 ATP mutant P17L, 37°C Solanum tuberosum
67
-
 ATP mutant P26L, 37°C Solanum tuberosum
72
-
 ATP mutant P66L, 37°C Solanum tuberosum
96
-
 ATP wild-type, 37°C Solanum tuberosum