Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.27 extracted from

  • Rudi, H.; Doan, D.N.P.; Olsen, O.A.
    A (His)6-tagged recombinant barley (Hordeum vulgare L.) endosperm ADP-glucose pyrophosphorylase expressed in the baculovirus-insect cell system is insensitive to allosteric regulation by 3-phosphoglycerate and inorganic phosphate (1997), FEBS Lett., 419, 124-130.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate recombinant enzyme is insensitive to 3-phosphoglycerate Hordeum vulgare

Cloned(Commentary)

Cloned (Comment) Organism
cloning of two isoforms, expressed in the baculovirus-insect cell system Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
phosphate recombinant enzyme is insensitive to phosphate Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000 + x * 55000 SDS-PAGE Hordeum vulgare
55000
-
x * 54000 + x * 55000 SDS-PAGE Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant enzyme after attachment of a histidine tag on only one of the subunits of a multimeric enzyme Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
Hordeum vulgare
-
seed
-
Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.6
-
-
Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
Hordeum vulgare diphosphate + ADP-glucose
-
?

Subunits

Subunits Comment Organism
heterotetramer x * 54000 + x * 55000 SDS-PAGE Hordeum vulgare