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Literature summary for 2.7.7.27 extracted from
- Purification and characterization of a thermostable ADP-glucose pyrophosphorylase from Thermus caldophilus GK-24 (1996), Biochem. J., 319, 977-983.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | - |
Escherichia coli |  |
| 3-phosphoglycerate | - |
Cereibacter sphaeroides | |
| 3-phosphoglycerate | - |
Rhodospirillum rubrum | |
| 3-phosphoglycerate | - |
Anabaena sp. | |
| 3-phosphoglycerate | minimal activation | Thermus caldophilus | |
| D-fructose 1,6-bisphosphate | - |
Escherichia coli | |
| D-fructose 1,6-bisphosphate | - |
Cereibacter sphaeroides | |
| D-fructose 1,6-bisphosphate | - |
Rhodospirillum rubrum | |
| D-fructose 1,6-bisphosphate | - |
Anabaena sp. | |
| D-fructose 1,6-bisphosphate | the most effective activator | Thermus caldophilus | |
| D-fructose 6-phosphate | - |
Escherichia coli | |
| D-fructose 6-phosphate | - |
Cereibacter sphaeroides | |
| D-fructose 6-phosphate | - |
Rhodospirillum rubrum | |
| D-fructose 6-phosphate | - |
Anabaena sp. | |
| D-fructose 6-phosphate | - |
Thermus caldophilus | |
| D-glucose 6-phosphate | - |
Escherichia coli | |
| D-glucose 6-phosphate | - |
Thermus caldophilus | |
| Phenylglyoxal | activation at pH 7 | Thermus caldophilus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermus caldophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Cereibacter sphaeroides | |
| ADP | - |
Escherichia coli | |
| ADP | - |
Rhodospirillum rubrum | |
| ADP | - |
Thermus caldophilus | |
| AMP | - |
Cereibacter sphaeroides | |
| AMP | - |
Escherichia coli | |
| AMP | - |
Rhodospirillum rubrum | |
| AMP | - |
Thermus caldophilus | |
| phosphate | - |
Anabaena sp. | |
| phosphate | - |
Cereibacter sphaeroides | |
| phosphate | - |
Escherichia coli | |
| phosphate | - |
Rhodospirillum rubrum | |
| phosphate | - |
Thermus caldophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
ATP | pH 7.6, 75°C | Thermus caldophilus | |
| 4.2 | - |
alpha-D-glucose 1-phosphate | pH 7.6, 75°C | Thermus caldophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Escherichia coli | |
| Mg2+ | requirement | Cereibacter sphaeroides | |
| Mg2+ | requirement | Rhodospirillum rubrum | |
| Mg2+ | requirement | Anabaena sp. | |
| Mg2+ | requirement | Thermus caldophilus | |
| additional information | Co2+ and Mn2+ are able to replace Mg2+, but not as effective as Mg2+ | Thermus caldophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition and comparison | Escherichia coli |
| additional information | - |
amino acid composition and comparison | Cereibacter sphaeroides |
| additional information | - |
amino acid composition and comparison | Rhodospirillum rubrum |
| additional information | - |
amino acid composition and comparison | Anabaena sp. |
| additional information | - |
amino acid composition and comparison | Thermus caldophilus |
| 52000 | - |
4 * 52000, SDS-PAGE | Thermus caldophilus |
| 230000 | - |
gel filtration | Thermus caldophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena sp. | - |
- |
- |
| Cereibacter sphaeroides | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Rhodospirillum rubrum | - |
- |
- |
| Thermus caldophilus | - |
GK-24 | - |
| Thermus caldophilus GK-24 | - |
GK-24 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus caldophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 161.7 | - |
- |
Thermus caldophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | - |
Cereibacter sphaeroides | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | - |
Rhodospirillum rubrum | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | - |
Anabaena sp. | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | not: ITP | Thermus caldophilus | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | no substrate are GTP, dATP, CTP | Thermus caldophilus | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | no substrate is UTP | Thermus caldophilus | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | not: ITP | Thermus caldophilus GK-24 | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | no substrate are GTP, dATP, CTP | Thermus caldophilus GK-24 | diphosphate + ADP-glucose | - |
r | |
| ATP + alpha-D-glucose 1-phosphate | no substrate is UTP | Thermus caldophilus GK-24 | diphosphate + ADP-glucose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Escherichia coli |
| homotetramer | - |
Cereibacter sphaeroides |
| homotetramer | - |
Rhodospirillum rubrum |
| homotetramer | - |
Anabaena sp. |
| homotetramer | 4 * 52000, SDS-PAGE | Thermus caldophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 73 | - |
- |
Thermus caldophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
30 min, 50% loss of activity | Thermus caldophilus |
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