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Literature summary for 2.7.7.27 extracted from

  • Meyer, C.R.; Borra, M.; Igarashi, R.; Lin, Y.S.; Springsteel, M.
    Characterization of ADP-glucose pyrophosphorylase from Rhodobacter sphaeroides 2.4.1: evidence for the involvement of arginine in allosteric regulation (1999), Arch. Biochem. Biophys., 372, 179-188.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
AMP
-
 Escherichia coli
AMP activation at pH 7 Cereibacter sphaeroides
D-fructose 1,6-bisphosphate
-
 Escherichia coli
D-fructose 1,6-bisphosphate
-
 Cereibacter sphaeroides
D-fructose 1,6-bisphosphate
-
 Anabaena sp.
D-fructose 6-phosphate
-
 Escherichia coli
D-fructose 6-phosphate
-
 Cereibacter sphaeroides
D-fructose 6-phosphate
-
 Anabaena sp.
pyruvate
-
 Cereibacter sphaeroides

Cloned(Commentary)

Cloned (Comment) Organism
-
 Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Cereibacter sphaeroides
ADP
-
 Escherichia coli
AMP AMP activation at pH 7, AMP acting as a competitive ligand for an allosteric site at pH 8 where both activators and inhibitors bind and mediate their effect Cereibacter sphaeroides
AMP
-
 Escherichia coli
phosphate
-
 Anabaena sp.
phosphate the presence of 2.5 mM D-fructose 6-phosphate completely prevents phosphate inhibition, in the presence of 1 mM phosphate, inhibition by phosphoenolpyruvate drops to 50% Cereibacter sphaeroides
phosphate
-
 Escherichia coli
phosphoenolpyruvate in the presence of 1 mM phosphate, inhibition by the 1 mM phosphoenolpyruvate drops to 50%, inhibition is reduced to 20% in the presence of 0.15 mM fructose 6-phosphate Cereibacter sphaeroides
phosphoenolpyruvate
-
 Escherichia coli
pyridoxal 5'-phosphate
-
 Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 alpha-D-glucose 1-phosphate pH 8, 37°C, in absence of fructose 6-phosphate Cereibacter sphaeroides
0.056
-
 alpha-D-glucose 1-phosphate pH 8, 37°C, in presence of fructose 6-phosphate Cereibacter sphaeroides
0.079
-
 ATP pH 8, 37°C, in presence of fructose 6-phosphate Cereibacter sphaeroides
0.22
-
 ATP pH 8, 37°C, enzyme modified for 30 min with 5 mM 2,3-butanedione Cereibacter sphaeroides
0.4
-
 ATP pH 8, 37°C, in absence of fructose 6-phosphate Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Escherichia coli
Mg2+ requirement Cereibacter sphaeroides
Mg2+ requirement Anabaena sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 N-terminal sequence and comparison Escherichia coli
additional information
-
 N-terminal sequence and comparison Cereibacter sphaeroides
additional information
-
 amino acid composition and comparison Escherichia coli
additional information
-
 amino acid composition and comparison Cereibacter sphaeroides
additional information
-
 amino acid composition and comparison Anabaena sp.
51000
-
 2 * 51000 Cereibacter sphaeroides
110000
-
 gel filtration Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
-
-
Cereibacter sphaeroides
-
-
-
Cereibacter sphaeroides Q9RNH7 2.4.1
-
Escherichia coli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification
-
 Escherichia coli
side-chain modification chemical modification of the purified enzyme with the arginyl reagents 2,3 butanedione and phenylglyoxal results in desensitation of the enzyme to both activators and inhibitors, affinity to ATP of enzyme modified 30 min with 5 mM 2,3-butanedione increases 3fold in comparison to the unmodified enzyme Cereibacter sphaeroides

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
172
-
-
 Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-80°C, stable for more than 3 months Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Escherichia coli diphosphate + ADP-glucose
-
 ?
ATP + alpha-D-glucose 1-phosphate
-
 Cereibacter sphaeroides diphosphate + ADP-glucose
-
 ?
ATP + alpha-D-glucose 1-phosphate
-
 Anabaena sp. diphosphate + ADP-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 51000 Cereibacter sphaeroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.12
-
 phosphoenolpyruvate pH 8.0, 37°C Cereibacter sphaeroides
0.37
-
 phosphate pH 8.0, 37°C Cereibacter sphaeroides
0.51
-
 ADP pH 8.0, 37°C Cereibacter sphaeroides