Literature summary for 2.7.7.27 extracted from

Yung, S.G.; Preiss, J.
Biosynthesis of bacterial glycogen: purification and structural properties of Rhodospirillum tenue adenosine diphosphate glucose synthetase (1981), J. Bacteriol., 147, 101-109.

General Stability

General Stability	Organism
no reducing reagent required during purification	Rhodocyclus tenuis
phosphate stabilizes	Rhodocyclus tenuis

Metals/Ions

Metals/Ions	Comment	Organism
Mg2+	requirement	Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+	requirement	Escherichia coli
Mg2+	requirement	Cereibacter sphaeroides
Mg2+	requirement	Spinacia oleracea
Mg2+	requirement	Rubrivivax gelatinosus
Mg2+	requirement	Rhodopseudomonas palustris
Mg2+	requirement	Rhodobacter capsulatus
Mg2+	requirement	Rhodospirillum rubrum
Mg2+	requirement	Rhodomicrobium vannielii
Mg2+	requirement	Magnetospirillum molischianum
Mg2+	requirement	Blastochloris viridis
Mg2+	requirement	Rhodoblastus acidophilus
Mg2+	requirement	Rhodopila globiformis
Mg2+	requirement	Rhodocyclus tenuis
Mg2+	requirement	Magnetospirillum fulvum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	-	Spinacia oleracea
additional information	-	-	Rubrivivax gelatinosus
additional information	-	-	Rhodopseudomonas palustris
additional information	-	-	Rhodobacter capsulatus
additional information	-	-	Rhodomicrobium vannielii
additional information	-	-	Magnetospirillum molischianum
additional information	-	-	Blastochloris viridis
additional information	-	-	Rhodoblastus acidophilus
additional information	-	-	Rhodopila globiformis
additional information	-	-	Magnetospirillum fulvum
additional information	-	N-terminal sequence and comparison	Salmonella enterica subsp. enterica serovar Typhimurium
additional information	-	N-terminal sequence and comparison	Escherichia coli
additional information	-	N-terminal sequence and comparison	Cereibacter sphaeroides
additional information	-	N-terminal sequence and comparison	Rhodospirillum rubrum
additional information	-	N-terminal sequence and comparison	Rhodocyclus tenuis
additional information	-	amino acid composition and comparison	Salmonella enterica subsp. enterica serovar Typhimurium
additional information	-	amino acid composition and comparison	Escherichia coli
additional information	-	amino acid composition and comparison	Cereibacter sphaeroides
additional information	-	amino acid composition and comparison	Rhodospirillum rubrum
additional information	-	amino acid composition and comparison	Rhodocyclus tenuis
51000	-	4 * 51000, SDS-PAGE	Escherichia coli
51000	-	4 * 51000, SDS-PAGE	Rhodocyclus tenuis
215000	-	sucrose density gradient centrifugation	Rhodocyclus tenuis

Organism

Organism	UniProt	Comment	Textmining
Blastochloris viridis	-	-	-
Cereibacter sphaeroides	-	strains 3244 and JT	-
Escherichia coli	-	-	-
Magnetospirillum fulvum	-	-	-
Magnetospirillum molischianum	-	-	-
Rhodobacter capsulatus	-	-	-
Rhodoblastus acidophilus	-	-	-
Rhodocyclus tenuis	-	-	-
Rhodomicrobium vannielii	-	-	-
Rhodopila globiformis	-	-	-
Rhodopseudomonas palustris	-	-	-
Rhodospirillum rubrum	-	-	-
Rubrivivax gelatinosus	-	-	-
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-
Spinacia oleracea	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rhodocyclus tenuis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
114	-	-	Rhodocyclus tenuis

Storage Stability

Storage Stability	Organism
0°C, in 30 mM potassium phosphate buffer, pH 7, 4 days	Rhodocyclus tenuis
4°C, 30-50% loss of activity overnight	Rhodocyclus tenuis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + alpha-D-glucose 1-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Escherichia coli	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Cereibacter sphaeroides	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Spinacia oleracea	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Rubrivivax gelatinosus	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Rhodopseudomonas palustris	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Rhodobacter capsulatus	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Rhodospirillum rubrum	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Rhodomicrobium vannielii	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Magnetospirillum molischianum	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Blastochloris viridis	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Rhodoblastus acidophilus	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Rhodopila globiformis	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Rhodocyclus tenuis	diphosphate + ADP-glucose	-	r
ATP + alpha-D-glucose 1-phosphate	-	Magnetospirillum fulvum	diphosphate + ADP-glucose	-	r

Subunits

Subunits	Comment	Organism
homotetramer	-	Salmonella enterica subsp. enterica serovar Typhimurium
homotetramer	-	Cereibacter sphaeroides
homotetramer	-	Spinacia oleracea
homotetramer	-	Rhodospirillum rubrum
homotetramer	4 * 51000, SDS-PAGE	Escherichia coli
homotetramer	4 * 51000, SDS-PAGE	Rhodocyclus tenuis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	-	4 days, stable in the presence of 30 mM potassium phosphate, pH 7	Rhodocyclus tenuis
37	-	4 days, stable in the presence of 30 mM potassium phosphate, pH 7	Rhodocyclus tenuis