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Literature summary for 2.7.7.27 extracted from
- Characterization of the kinetic, regulatory, and structural properties of ADP-glucose pyrophosphorylase from Chlamydomonas reinhardtii (1994), Plant Physiol., 104, 1287-1294.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | allosteric activation | Chlamydomonas reinhardtii |  |
| 3-phosphoglycerate | activation | Chlamydomonas reinhardtii | |
| D-fructose 1,6-bisphosphate | - |
Chlamydomonas reinhardtii | |
| D-fructose 6-phosphate | activation | Chlamydomonas reinhardtii | |
| D-fructose 6-phosphate | slight activation | Chlamydomonas reinhardtii | |
| D-Glucose 1,6-bisphosphate | slight activation | Chlamydomonas reinhardtii | |
| D-glucose 6-phosphate | activation | Chlamydomonas reinhardtii | |
| D-glucose 6-phosphate | slight activation | Chlamydomonas reinhardtii | |
| NADPH | activation | Chlamydomonas reinhardtii | |
| NADPH | activation, slight | Chlamydomonas reinhardtii | |
| phosphoenolpyruvate | activation | Chlamydomonas reinhardtii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 1 mM | Chlamydomonas reinhardtii | |
| diphosphate | substrate inhibition, 1 mM | Chlamydomonas reinhardtii | |
| phosphate | allosteric inhibitor | Chlamydomonas reinhardtii | |
| Rabbit antiserum raised against spinach enzyme | not antiserum against E. coli enzyme | Chlamydomonas reinhardtii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, kinetic study | Chlamydomonas reinhardtii | |
| 0.48 | - |
ATP | pH 8.0, 37°C, kinetic study | Chlamydomonas reinhardtii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000 + x * 53000 SDS-PAGE | Chlamydomonas reinhardtii |
| 53000 | - |
x * 50000 + x * 53000 SDS-PAGE | Chlamydomonas reinhardtii |
| 210000 | - |
gel filtration | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | Chlamydomonas reinhardtii | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
green alga, strain 137C | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 81 | - |
- |
Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Chlamydomonas reinhardtii | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Chlamydomonas reinhardtii | diphosphate + ADP-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | x * 50000 + x * 53000 SDS-PAGE | Chlamydomonas reinhardtii |
| homotetramer | - |
Chlamydomonas reinhardtii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, inactivation | Chlamydomonas reinhardtii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.054 | - |
phosphate | pH 8.0, 37°C, in absence of 3-phosphoglycerate | Chlamydomonas reinhardtii | |
| 0.2 | - |
phosphate | pH 8.0, 37°C, in presence of 0.25 mM 3-phosphoglycerate | Chlamydomonas reinhardtii | |
| 0.53 | - |
phosphate | pH 8.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate | Chlamydomonas reinhardtii | |
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