Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | allosteric activation | Spinacia oleracea | |
3-phosphoglycerate | activation | Spinacia oleracea | |
D-fructose 1,6-bisphosphate | activation of pyrophosphorolysis | Spinacia oleracea | |
D-fructose 1,6-bisphosphate | activation of ADP-glucose synthesis | Spinacia oleracea | |
D-fructose 6-phosphate | activation | Spinacia oleracea | |
D-fructose 6-phosphate | activation of ADPglucose synthesis | Spinacia oleracea | |
phosphoenolpyruvate | activation | Spinacia oleracea | |
phosphoenolpyruvate | activation of pyrophosphorolysis | Spinacia oleracea | |
phosphoenolpyruvate | ADPglucose synthesis | Spinacia oleracea |
General Stability | Organism |
---|---|
polyvinylpyrrolidone, i.e. PVP fractionation stabilizes during purification, 20% sucrose and 30 mM phosphate stabilize during purification | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | allosteric inhibitor | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
alpha-D-glucose 1-phosphate | pH 7.3, 37°C, in presence of 1 mM 3-phosphoglycerate | Spinacia oleracea | |
0.045 | - |
ATP | pH 7.3, 37°C, in presence of 1 mM 3-phosphoglycerate | Spinacia oleracea | |
0.105 | - |
alpha-D-glucose 1-phosphate | pH 7.3, 37°C, in absence of 3-phosphoglycerate | Spinacia oleracea | |
0.32 | - |
ATP | pH 7.3, 37°C, in absence of 3-phosphoglycerate | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Spinacia oleracea | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000 + x * 48000, SDS-PAGE | Spinacia oleracea |
48000 | - |
x * 44000 + x * 48000, SDS-PAGE | Spinacia oleracea |
206000 | - |
sucrose density gradient ultracentrifugation | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | Spinacia oleracea | one of the main regulatory steps in starch biosynthesis in plants | diphosphate + ADP-glucose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
156 | - |
- |
Spinacia oleracea |
Storage Stability | Organism |
---|---|
4°C, in 50 mM HEPES buffer, pH 7, 20% sucrose, 0.2 mM dithioerythritol, 1 mM EDTA, 2 weeks, about 60% loss of activity within 1 month | Spinacia oleracea |
frozen or 4°C, most stable in buffer containing 20% sucrose and 30 mM phosphate | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Spinacia oleracea | diphosphate + ADP-glucose | - |
r | |
ATP + alpha-D-glucose 1-phosphate | one of the main regulatory steps in starch biosynthesis in plants | Spinacia oleracea | diphosphate + ADP-glucose | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | x * 44000 + x * 48000, SDS-PAGE | Spinacia oleracea |
homotetramer | - |
Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
phosphate | pH 8.0, 37°C, in absence of 3-phosphoglycerate | Spinacia oleracea | |
0.064 | - |
phosphate | pH 7.3, 37°C, in absence of phosphoglycerate | Spinacia oleracea | |
0.97 | - |
phosphate | pH 7.3, 37°C, in presence of 3-phosphoglycerate | Spinacia oleracea | |
1.27 | - |
phosphate | pH 7.3, 37°C, in presence of 3-phosphoglycerate | Spinacia oleracea |