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Literature summary for 2.7.7.24 extracted from
- The structure of glucose-1-phosphate thymidylyltransferase from Mycobacterium tuberculosis reveals the location of an essential magnesium ion in the RmlA-type enzymes (2018), Protein Sci., 27, 441-450 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta2(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| enzyme in complex with either dTTP or dTDP-alpha-D-glucose, hanging drop vapor diffusion method, using either 18-22% (w/v) poly(ethylene glycol) 8000 and 100 mM MOPS (pH 7.0) or 20% (w/v) poly(ethylene glycol) 3350, 200 mM KCl and 100 mM CHES (pH 9.0), respectively | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0106 | - |
dTTP | at pH 7.5 and 25°C | Mycobacterium tuberculosis |  |
| 0.0327 | - |
alpha-D-glucose 1-phosphate | at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | in the RmlA/dTTP complex two Mg2+ are observed binding to the nucleotide | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + alpha-D-glucose 1-phosphate | Mycobacterium tuberculosis | - |
diphosphate + dTDP-alpha-D-glucose | - |
? | |
| dTTP + alpha-D-glucose 1-phosphate | Mycobacterium tuberculosis H37Rv | - |
diphosphate + dTDP-alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WH13 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WH13 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis | diphosphate + dTDP-alpha-D-glucose | - |
? | |
| dTTP + alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + dTDP-alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucose-1-phosphate thymidylyltransferase | - |
Mycobacterium tuberculosis |
| RmlA | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.3 | - |
alpha-D-glucose 1-phosphate | at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
| 7.9 | - |
dTTP | at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme catalyzes the first step in the biosynthesis of L-rhamnose, which is a deoxysugar critical for the integrity of the bacterium's cell wall | Mycobacterium tuberculosis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 220 | - |
alpha-D-glucose 1-phosphate | at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
| 750 | - |
dTTP | at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
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