Literature summary for 2.7.7.24 extracted from

Baumgartner, J.; Lee, J.; Halavaty, A.; Minasov, G.; Anderson, W.; Kuhn, M.
Structure of the Bacillus anthracis dTDP-l-rhamnose-biosynthetic enzyme glucose-1-phosphate thymidylyltransferase (RfbA) (2017), Acta Crystallogr. Sect. F, 73, 621-628 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) Magic cells	Bacillus anthracis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with dTDP-alpha-D-glucose and diphosphate, sitting drop vapor diffusion method, using 0.2 M sodium sulfate, 0.1 M bis-Tris propane pH 8.5, 20% (w/v) PEG 3350	Bacillus anthracis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	absolutely required for catalysis	Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dTTP + alpha-D-glucose 1-phosphate	Bacillus anthracis	-	diphosphate + dTDP-alpha-D-glucose	-	?
dTTP + alpha-D-glucose 1-phosphate	Bacillus anthracis Ames	-	diphosphate + dTDP-alpha-D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus anthracis	A0A4Y1W0P1	-	-
Bacillus anthracis Ames	A0A4Y1W0P1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Bacillus anthracis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dTTP + alpha-D-glucose 1-phosphate	-	Bacillus anthracis	diphosphate + dTDP-alpha-D-glucose	-	?
dTTP + alpha-D-glucose 1-phosphate	-	Bacillus anthracis Ames	diphosphate + dTDP-alpha-D-glucose	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Bacillus anthracis

Synonyms

Synonyms	Comment	Organism
dTDP-alpha-D-glucose pyrophosphorylase	-	Bacillus anthracis
Glc1P-thymidylyltransferase	-	Bacillus anthracis
glucose-1-phosphate thymidylyltransferase	-	Bacillus anthracis
RfbA	-	Bacillus anthracis
RmlA	-	Bacillus anthracis

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Release 2023.1 (January 2023)