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Literature summary for 2.7.7.24 extracted from
- Identification of an extremely thermostable enzyme with dual sugar-1-phosphate nucleotidylyltransferase activities from an acidothermophilic archaeon, Sulfolobus tokodaii strain 7 (2005), J. Biol. Chem., 280, 9698-9705 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21-Codon Plus (DE3)-RIL cells | Sulfurisphaera tokodaii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | analysis of a deletion mutant lacking the 170-residue C-terminal domain indicated that this region has an important role in the thermostability and activity of the protein. Specific initial velocity (glucose-1-phosphate thymidylyltransferase activity) is 23 times lower than that of the native enzyme when measured at 37°C | Sulfurisphaera tokodaii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
dTTP | pH 8.0, 80°C | Sulfurisphaera tokodaii |  |
| 0.05 | - |
dTDP-alpha-D-glucose | pH 8.0, 80°C | Sulfurisphaera tokodaii | |
| 0.39 | - |
diphosphate | pH 8.0, 80°C | Sulfurisphaera tokodaii | |
| 1.12 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 80°C | Sulfurisphaera tokodaii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+ | Sulfurisphaera tokodaii | |
| Mg2+ | absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+. The optimal Mg2+ concentration is 6 mM, but the enzymatic activity does not change substantially between 2 and 12 mM | Sulfurisphaera tokodaii | |
| Mn2+ | absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+ | Sulfurisphaera tokodaii | |
| Zn2+ | absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+ | Sulfurisphaera tokodaii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + alpha-D-glucose 1-phosphate | Sulfurisphaera tokodaii | the enzyme is involved in biosynthesis of L-rhamnose | diphosphate + dTDP-alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | Q975F9 | - |
- |
| Sulfurisphaera tokodaii DSM 16993 | Q975F9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + dTDP-alpha-D-glucose | - |
Sulfurisphaera tokodaii | dTTP + alpha-D-glucose 1-phosphate | - |
r | |
| dTTP + alpha-D-glucose 1-phosphate | - |
Sulfurisphaera tokodaii | diphosphate + dTDP-alpha-D-glucose | - |
r | |
| dTTP + alpha-D-glucose 1-phosphate | the enzyme is involved in biosynthesis of L-rhamnose | Sulfurisphaera tokodaii | diphosphate + dTDP-alpha-D-glucose | - |
? | |
| dTTP + alpha-D-glucose 1-phosphate | - |
Sulfurisphaera tokodaii DSM 16993 | diphosphate + dTDP-alpha-D-glucose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucose-1-phosphate thymidylyltransferase | - |
Sulfurisphaera tokodaii |
| ST0452 | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Sulfurisphaera tokodaii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 100 | 70°C: about 40% of maximal activity, 100°C: about 80% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life: 180 min | Sulfurisphaera tokodaii |
| 95 | - |
half-life: 60 min | Sulfurisphaera tokodaii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Sulfurisphaera tokodaii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity | Sulfurisphaera tokodaii |
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